Detailed information of TA system TA058397

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 382751..383366
• Replicon: chromosome
• Accession: NZ_CP013318
• Organism: Vibrio cholerae strain NCTC5395

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: ASZ85_RS16065
• Protein ID: WP_001162670.1
• Coordinates: 382751..383038 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ASZ85_RS16070
• Protein ID: WP_001232701.1
• Coordinates: 383049..383366 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ85_RS16020	378243..378500	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ85_RS16025	378488..378790	+	303	WP_000229318.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ85_RS16030	379079..379303	+	225	WP_001894423.1	DUF3709 domain-containing protein	-
ASZ85_RS16035	379747..379866	+	120	WP_071919600.1	DUF645 family protein	-
ASZ85_RS21000	379870..379923	+	54	Protein_360	DUF645 family protein	-
ASZ85_RS16050	380525..381529	+	1005	WP_000964931.1	LD-carboxypeptidase	-
ASZ85_RS16060	382304..382484	+	181	Protein_362	DUF645 family protein	-
ASZ85_RS16065	382751..383038	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ASZ85_RS16070	383049..383366	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
ASZ85_RS21445	383363..383473	+	111	WP_134814058.1	DUF3265 domain-containing protein	-
ASZ85_RS16080	383519..384058	+	540	WP_000099372.1	hypothetical protein	-
ASZ85_RS16085	384203..384637	+	435	WP_000453680.1	GNAT family N-acetyltransferase	-
ASZ85_RS16090	384864..385142	+	279	WP_001921601.1	DUF3709 domain-containing protein	-
ASZ85_RS21020	385394..385600	+	207	Protein_369	DUF3709 domain-containing protein	-
ASZ85_RS21025	385800..385901	+	102	WP_001921603.1	hypothetical protein	-
ASZ85_RS16095	385891..386277	+	387	WP_000703163.1	VOC family protein	-
ASZ85_RS16100	386472..386723	+	252	WP_001890111.1	TIGR03643 family protein	-
ASZ85_RS16105	386696..386845	+	150	WP_071908333.1	DUF3265 domain-containing protein	-
ASZ85_RS16110	387058..387210	+	153	WP_001884520.1	DUF645 family protein	-
ASZ85_RS16115	387411..387782	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
ASZ85_RS16120	388078..388260	+	183	WP_001900194.1	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	338285..496833	158548
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.009	inside	Integron	-	-	343365..496457	153092

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T58397 WP_001162670.1 NZ_CP013318:382751-383038 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T58397 NZ_CP013318:382751-383038 [Vibrio cholerae]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT58397 WP_001232701.1 NZ_CP013318:383049-383366 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT58397 NZ_CP013318:383049-383366 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36