56915

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 358082..358697 Replicon chromosome
Accession NZ_CP012666
Organism Vibrio cholerae strain ICDC-VC661

Toxin (Protein)


Gene name higB Uniprot ID Q9KMG5
Locus tag AN947_RS01905 Protein ID WP_001162670.1
Coordinates 358082..358369 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag AN947_RS01910 Protein ID WP_001232701.1
Coordinates 358380..358697 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AN947_RS20750 353718..353813 + 96 WP_001907607.1 DUF645 family protein -
AN947_RS01865 354007..354324 - 318 WP_001180243.1 type II toxin-antitoxin system RelE/ParE family toxin -
AN947_RS01870 354343..354585 - 243 WP_000557292.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
AN947_RS01875 354827..355336 + 510 WP_000405987.1 GNAT family N-acetyltransferase -
AN947_RS01880 355393..356046 + 654 WP_000226874.1 hypothetical protein -
AN947_RS01885 356356..356574 + 219 WP_001917086.1 DUF3709 domain-containing protein -
AN947_RS01895 356977..357210 + 234 WP_032482776.1 DUF3709 domain-containing protein -
AN947_RS01900 357635..357815 + 181 Protein_344 DUF645 family protein -
AN947_RS01905 358082..358369 + 288 WP_001162670.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AN947_RS01910 358380..358697 + 318 WP_001232701.1 HigA family addiction module antidote protein Antitoxin
AN947_RS20755 358694..358804 + 111 WP_134814058.1 DUF3265 domain-containing protein -
AN947_RS01920 358981..359106 + 126 WP_001944767.1 DUF645 family protein -
AN947_RS20760 359122..359160 + 39 WP_106019119.1 hypothetical protein -
AN947_RS01930 359373..360377 + 1005 WP_000964925.1 LD-carboxypeptidase -
AN947_RS01935 360530..360976 + 447 WP_000128977.1 hypothetical protein -
AN947_RS01940 361096..362025 + 930 WP_000335802.1 hypothetical protein -
AN947_RS01945 362022..362132 + 111 WP_071917832.1 DUF3265 domain-containing protein -
AN947_RS01950 362160..362636 + 477 WP_001047180.1 GNAT family N-acetyltransferase -
AN947_RS01955 362773..363288 + 516 WP_001201532.1 lipocalin family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 304645..399892 95247
inside Integron - - 309725..399516 89791


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(2-95)

Antitoxin

Cro/C1-type helix-turn-helix domain

(12-69)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11349.95 Da        Isoelectric Point: 6.7187

>T56915 WP_001162670.1 NZ_CP012666:358082-358369 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Download         Length: 288 bp

>T56915 NZ_CP012666:358082-358369 [Vibrio cholerae]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11705.63 Da        Isoelectric Point: 10.4711

>AT56915 WP_001232701.1 NZ_CP012666:358380-358697 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Download         Length: 318 bp

>AT56915 NZ_CP012666:358380-358697 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F2HI36

Antitoxin

Source ID Structure

References