Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/YafQ-RelB
Location 54322..54862 Replicon chromosome
Accession NZ_CP007635
Organism Vibrio cholerae strain 2012EL-2176

Toxin (Protein)


Gene name relE Uniprot ID A0A151JGK8
Locus tag EN18_RS14435 Protein ID WP_000277238.1
Coordinates 54322..54591 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q9KML3
Locus tag EN18_RS14440 Protein ID WP_001258569.1
Coordinates 54584..54862 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EN18_RS14395 49543..50079 + 537 WP_000469482.1 GNAT family N-acetyltransferase -
EN18_RS14400 50216..50731 + 516 WP_001201509.1 lipocalin family protein -
EN18_RS14405 50881..51378 - 498 WP_000982260.1 GNAT family N-acetyltransferase -
EN18_RS14410 51375..51647 - 273 WP_000246253.1 DUF1778 domain-containing protein -
EN18_RS21085 52040..52165 + 126 WP_001900195.1 DUF645 family protein -
EN18_RS21090 52181..52219 + 39 WP_106019118.1 hypothetical protein -
EN18_RS14425 52415..52861 + 447 WP_000006157.1 hypothetical protein -
EN18_RS14430 52924..54135 + 1212 WP_001122813.1 IS256 family transposase -
EN18_RS14435 54322..54591 - 270 WP_000277238.1 type II toxin-antitoxin system YafQ family toxin Toxin
EN18_RS14440 54584..54862 - 279 WP_001258569.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
EN18_RS20510 55148..55426 + 279 WP_001921601.1 DUF3709 domain-containing protein -
EN18_RS21105 55678..55884 + 207 Protein_69 DUF3709 domain-containing protein -
EN18_RS21110 56084..56185 + 102 WP_001921603.1 hypothetical protein -
EN18_RS14450 56175..56561 + 387 WP_000703163.1 VOC family protein -
EN18_RS14455 56756..57007 + 252 WP_001890111.1 TIGR03643 family protein -
EN18_RS20520 56980..57129 + 150 WP_071908333.1 DUF3265 domain-containing protein -
EN18_RS14460 57221..57463 + 243 WP_000107461.1 hypothetical protein -
EN18_RS14465 57715..57993 + 279 WP_000280324.1 DUF3709 domain-containing protein -
EN18_RS21540 58368..58511 + 144 Protein_76 DUF645 family protein -
EN18_RS21545 58565..58603 + 39 WP_082798268.1 hypothetical protein -
EN18_RS14475 58815..59282 + 468 WP_001289288.1 OsmC family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 31037..137542 106505
inside Integron - - 36117..137166 101049
- flank IS/Tn - - 52924..54135 1211


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(2-89)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10406.11 Da        Isoelectric Point: 9.5084

>T46751 WP_000277238.1 NZ_CP007635:c54591-54322 [Vibrio cholerae]
MYKLEYSTQFKKDFKKITKMPISDIIEVGNVISKLQRGEKLEPKNVDHPLTGNWVGFRDCHIKPDLVLIYRVFNDQLQLA
RIGSHSDLF

Download         Length: 270 bp

>T46751 NZ_CP007635:c54591-54322 [Vibrio cholerae]
ATGTATAAACTCGAATACTCCACACAGTTTAAGAAAGATTTCAAAAAGATAACTAAGATGCCGATTTCAGACATCATTGA
AGTCGGCAATGTTATTTCTAAGCTGCAACGCGGCGAAAAGCTTGAACCCAAGAATGTTGACCATCCGTTAACTGGAAATT
GGGTTGGATTTCGAGACTGCCACATTAAACCCGACCTAGTGTTAATCTATCGAGTTTTTAACGATCAACTACAACTAGCG
CGCATTGGTTCACATAGTGATTTATTCTAA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10088.58 Da        Isoelectric Point: 6.0728

>AT46751 WP_001258569.1 NZ_CP007635:c54862-54584 [Vibrio cholerae]
MRTEMLSTRIDHDTKIAFTNVCDEMGLSTSQAIKLFAKAVINHGGIPFELRVPQPNEVTASAIQELVEGKGHKAESVEAM
LNELTEGKVKHV

Download         Length: 279 bp

>AT46751 NZ_CP007635:c54862-54584 [Vibrio cholerae]
ATGAGAACAGAAATGCTGAGCACGCGCATTGACCACGACACGAAAATTGCGTTCACAAACGTCTGCGATGAGATGGGTCT
AAGTACATCACAGGCCATAAAGTTATTTGCAAAAGCGGTTATTAATCATGGTGGAATCCCTTTTGAGCTGCGAGTTCCAC
AGCCTAATGAGGTTACTGCATCTGCAATTCAAGAGCTCGTTGAAGGAAAAGGACATAAAGCTGAATCCGTTGAAGCTATG
CTGAATGAGCTTACTGAAGGCAAAGTTAAGCATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A151JGK8

Antitoxin

Source ID Structure
AlphaFold DB A0A151KTP9

References