Detailed information of TA system TA022076

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-GNAT
• Location: 835506..836599
• Replicon: chromosome
• Accession: NC_009456
• Organism: Vibrio cholerae O395

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9KMA2
• Locus tag: VC0395_RS03825
• Protein ID: WP_000351248.1
• Coordinates: 835506..835907 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: VC0395_RS03830
• Protein ID: WP_001047169.1
• Coordinates: 835907..836599 (-)
• Length: 231 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VC0395_RS03790	830644..832392	+	1749	WP_000063386.1	hypothetical protein	-
VC0395_RS03795	832605..832850	+	246	WP_001912198.1	hypothetical protein	-
VC0395_RS03800	833106..833258	-	153	WP_001884520.1	DUF645 family protein	-
VC0395_RS03805	833580..834035	-	456	WP_001245327.1	GNAT family N-acetyltransferase	-
VC0395_RS03810	834163..834450	-	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
VC0395_RS03815	834440..834691	-	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
VC0395_RS03820	834905..835318	-	414	WP_000049417.1	VOC family protein	-
VC0395_RS20535	835393..835536	-	144	WP_071908341.1	DUF3265 domain-containing protein	-
VC0395_RS03825	835506..835907	-	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
VC0395_RS03830	835907..836599	-	693	WP_001047169.1	GNAT family N-acetyltransferase	Antitoxin
VC0395_RS03835	836736..837042	-	307	Protein_739	CatB-related O-acetyltransferase	-
VC0395_RS03840	837110..838090	+	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
VC0395_RS20945	838215..838370	-	156	WP_000751734.1	hypothetical protein	-
VC0395_RS03850	838538..838972	-	435	WP_000256036.1	GNAT family N-acetyltransferase	-
VC0395_RS03855	839109..839423	-	315	WP_000071008.1	DNA-binding transcriptional regulator	-
VC0395_RS03860	839410..839742	-	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	-
VC0395_RS03870	840083..840310	-	228	WP_001894457.1	DUF1289 domain-containing protein	-
VC0395_RS21050	840259..840373	-	115	Protein_746	acetyltransferase	-
VC0395_RS21055	840490..840661	-	172	Protein_747	DUF645 family protein	-
VC0395_RS03875	840979..841248	-	270	WP_001198131.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	833458..916350	82892
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	837110..838090	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-132)

Antitoxin

• GNAT domain (36-153)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14608.71 Da
• Isoelectric Point: 4.0128

>T22076 WP_000351248.1 NC_009456:c835907-835506 [Vibrio cholerae O395]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Nucleotide

• Length: 402 bp

>T22076 NC_009456:c835907-835506 [Vibrio cholerae O395]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin

Protein

• Length: 231 a.a.
• Molecular weight: 26352.73 Da
• Isoelectric Point: 8.5633

>AT22076 WP_001047169.1 NC_009456:c836599-835907 [Vibrio cholerae O395]
MNLEEFQESDFDLLIKWIDSDELNYLWGCPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 693 bp

>AT22076 NC_009456:c836599-835907 [Vibrio cholerae O395]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTTGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTATGGTTGTTGTGATTGAGTTTGGCGGTATGCGTTGTCAGCCCCTTAGGCGGGCGTTATGTTTTTTATCACGTTTT
GGGGCTATAATCGGCCTAACCTTTATTGATAGTAGGTGTGATATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGT
TGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGACTGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTA
AACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGGCTGATATGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q0PN34