95382

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 94424..95039 Replicon chromosome
Accession NZ_CP026648
Organism Vibrio cholerae O1 biovar El Tor strain HC1037

Toxin (Protein)


Gene name higB Uniprot ID Q9KMG5
Locus tag C4E16_RS14895 Protein ID WP_001162670.1
Coordinates 94424..94711 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag C4E16_RS14900 Protein ID WP_001232701.1
Coordinates 94722..95039 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4E16_RS19565 90060..90155 + 96 WP_001907607.1 DUF645 family protein -
C4E16_RS14855 90349..90666 - 318 WP_001180243.1 type II toxin-antitoxin system RelE/ParE family toxin -
C4E16_RS14860 90685..90927 - 243 WP_000557292.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
C4E16_RS14865 91169..91678 + 510 WP_000405987.1 GNAT family N-acetyltransferase -
C4E16_RS14870 91735..92388 + 654 WP_000226874.1 hypothetical protein -
C4E16_RS14875 92698..92916 + 219 WP_001917086.1 DUF3709 domain-containing protein -
C4E16_RS14880 93319..93552 + 234 WP_032482776.1 DUF3709 domain-containing protein -
C4E16_RS14885 93977..94157 + 181 Protein_139 DUF645 family protein -
C4E16_RS14895 94424..94711 + 288 WP_001162670.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C4E16_RS14900 94722..95039 + 318 WP_001232701.1 HigA family addiction module antidote protein Antitoxin
C4E16_RS19570 95036..95146 + 111 WP_134814058.1 DUF3265 domain-containing protein -
C4E16_RS14915 95323..95448 + 126 WP_001944767.1 DUF645 family protein -
C4E16_RS19440 95464..95502 + 39 WP_106019119.1 hypothetical protein -
C4E16_RS14925 95715..96719 + 1005 WP_000964925.1 LD-carboxypeptidase -
C4E16_RS14930 96872..97318 + 447 WP_000128977.1 hypothetical protein -
C4E16_RS14935 97438..98367 + 930 WP_000335802.1 hypothetical protein -
C4E16_RS14940 98364..98474 + 111 WP_071917832.1 DUF3265 domain-containing protein -
C4E16_RS14945 98502..98978 + 477 WP_001047180.1 GNAT family N-acetyltransferase -
C4E16_RS14950 99115..99630 + 516 WP_001201528.1 lipocalin family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 31043..136234 105191
inside Integron - - 36123..135858 99735


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(2-95)

Antitoxin

Cro/C1-type helix-turn-helix domain

(12-69)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11349.95 Da        Isoelectric Point: 6.7187

>T95382 WP_001162670.1 NZ_CP026648:94424-94711 [Vibrio cholerae O1 biovar El Tor]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Download         Length: 288 bp

>T95382 NZ_CP026648:94424-94711 [Vibrio cholerae O1 biovar El Tor]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11705.63 Da        Isoelectric Point: 10.4711

>AT95382 WP_001232701.1 NZ_CP026648:94722-95039 [Vibrio cholerae O1 biovar El Tor]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Download         Length: 318 bp

>AT95382 NZ_CP026648:94722-95039 [Vibrio cholerae O1 biovar El Tor]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F2HI36

Antitoxin

Source ID Structure

References