Detailed information of TA system TA058365

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 454488..455254
• Replicon: chromosome
• Accession: NZ_CP013314
• Organism: Vibrio cholerae strain E9120

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9K2P7
• Locus tag: ASZ79_RS16940
• Protein ID: WP_000982260.1
• Coordinates: 454488..454985 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9K2J6
• Locus tag: ASZ79_RS16945
• Protein ID: WP_000246253.1
• Coordinates: 454982..455254 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ79_RS16890	450350..450656	+	307	Protein_499	CatB-related O-acetyltransferase	-
ASZ79_RS16895	450793..451485	+	693	WP_001047169.1	GNAT family N-acetyltransferase	-
ASZ79_RS16900	451485..451886	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
ASZ79_RS20750	451856..451999	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
ASZ79_RS16910	452074..452487	+	414	WP_000049417.1	VOC family protein	-
ASZ79_RS16915	452701..452952	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ79_RS16920	452942..453229	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ79_RS16925	453357..453812	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
ASZ79_RS16935	454134..454286	+	153	WP_001884520.1	DUF645 family protein	-
ASZ79_RS16940	454488..454985	-	498	WP_000982260.1	GNAT family N-acetyltransferase	Toxin
ASZ79_RS16945	454982..455254	-	273	WP_000246253.1	DUF1778 domain-containing protein	Antitoxin
ASZ79_RS16950	455485..456153	+	669	WP_000043871.1	hypothetical protein	-
ASZ79_RS16955	456305..456592	+	288	WP_000426470.1	hypothetical protein	-
ASZ79_RS16960	456733..457104	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
ASZ79_RS16965	457171..457596	+	426	WP_001882332.1	DUF1778 domain-containing protein	-
ASZ79_RS16970	457593..458126	+	534	WP_000118351.1	GNAT family N-acetyltransferase	-
ASZ79_RS16975	458328..458585	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ79_RS16980	458573..458875	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ79_RS16985	459109..460026	+	918	WP_000186333.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304700..468464	163764
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.005	inside	Integron	-	-	309780..468088	158308
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	449302..450282	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-86)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18527.18 Da
• Isoelectric Point: 8.7753

>T58365 WP_000982260.1 NZ_CP013314:c454985-454488 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Nucleotide

• Length: 498 bp

>T58365 NZ_CP013314:c454985-454488 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9683.22 Da
• Isoelectric Point: 6.2988

>AT58365 WP_000246253.1 NZ_CP013314:c455254-454982 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Nucleotide

• Length: 273 bp

>AT58365 NZ_CP013314:c455254-454982 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Structures

Toxin

• AlphaFold DB: A0A0Q0KGB1

Antitoxin

• AlphaFold DB: A0A0F2IC79