Detailed information of TA system TA064439

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 433768..434401
• Replicon: chromosome
• Accession: NZ_CP016325
• Organism: Vibrio cholerae 2740-80

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMA6
• Locus tag: BBB50_RS16125
• Protein ID: WP_000843587.1
• Coordinates: 433768..434100 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BBB50_RS16130
• Protein ID: WP_000071008.1
• Coordinates: 434087..434401 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBB50_RS20525	429209..429412	+	204	WP_001911745.1	hypothetical protein	-
BBB50_RS20335	429693..429866	+	174	WP_001882305.1	DUF3709 domain-containing protein	-
BBB50_RS16095	430226..430495	+	270	WP_001198131.1	hypothetical protein	-
BBB50_RS20585	430813..430984	+	172	Protein_452	DUF645 family protein	-
BBB50_RS16100	431193..431579	+	387	WP_000703163.1	VOC family protein	-
BBB50_RS16105	431781..432023	+	243	WP_000107461.1	hypothetical protein	-
BBB50_RS16110	432194..432571	+	378	WP_000411109.1	hypothetical protein	-
BBB50_RS20540	432628..432742	+	115	Protein_456	acetyltransferase	-
BBB50_RS16115	432691..432972	+	282	WP_001894453.1	DUF1289 domain-containing protein	-
BBB50_RS19980	433138..433251	+	114	WP_001889158.1	hypothetical protein	-
BBB50_RS19985	433200..433427	+	228	WP_001894457.1	DUF1289 domain-containing protein	-
BBB50_RS16125	433768..434100	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BBB50_RS16130	434087..434401	+	315	WP_000071008.1	DNA-binding transcriptional regulator	Antitoxin
BBB50_RS16135	434538..434972	+	435	WP_000256036.1	GNAT family N-acetyltransferase	-
BBB50_RS16140	435140..435295	+	156	WP_000751734.1	hypothetical protein	-
BBB50_RS16145	435420..436400	-	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
BBB50_RS16150	436468..436774	+	307	Protein_465	CatB-related O-acetyltransferase	-
BBB50_RS16155	436911..437603	+	693	WP_001047169.1	GNAT family N-acetyltransferase	-
BBB50_RS16160	437603..438004	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
BBB50_RS20340	437974..438117	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
BBB50_RS16165	438192..438605	+	414	WP_000049417.1	VOC family protein	-
BBB50_RS16170	438819..439070	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
BBB50_RS16175	439060..439347	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	304243..451810	147567
	-	inside	Integron	-	-	309323..451434	142111
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	435420..436400	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-92)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 13005.95 Da
• Isoelectric Point: 9.9244

>T64439 WP_000843587.1 NZ_CP016325:433768-434100 [Vibrio cholerae 2740-80]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS

Nucleotide

• Length: 333 bp

>T64439 NZ_CP016325:433768-434100 [Vibrio cholerae 2740-80]
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGGCGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11696.20 Da
• Isoelectric Point: 6.7600

>AT64439 WP_000071008.1 NZ_CP016325:434087-434401 [Vibrio cholerae 2740-80]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL

Nucleotide

• Length: 315 bp

>AT64439 NZ_CP016325:434087-434401 [Vibrio cholerae 2740-80]
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCGCGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA

Structures

Toxin

• PDB: 5JA9
• PDB: 8A0X
• PDB: 5MJE
• PDB: 5JA8
• PDB: 5JAA