Detailed information of TA system TA074618

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 759425..760380
• Replicon: chromosome
• Accession: NZ_CP020407
• Organism: Vibrio cholerae strain FDAARGOS_223

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KM94
• Locus tag: A6J62_RS03455
• Protein ID: WP_000118351.1
• Coordinates: 759425..759958 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: A6J62_RS19635
• Protein ID: WP_001882332.1
• Coordinates: 759955..760380 (-)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6J62_RS03410	754749..755621	-	873	WP_000254716.1	DUF3800 domain-containing protein	-
A6J62_RS03415	755796..756014	-	219	WP_071908318.1	GNAT family N-acetyltransferase	-
A6J62_RS03420	756078..756515	-	438	WP_000503169.1	IS200/IS605-like element IS1004 family transposase	-
A6J62_RS03425	756634..756843	-	210	Protein_647	GNAT family N-acetyltransferase	-
A6J62_RS03430	756979..757368	-	390	WP_001081302.1	hypothetical protein	-
A6J62_RS03435	757525..758442	-	918	WP_000186333.1	alpha/beta hydrolase	-
A6J62_RS03440	758676..758978	-	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
A6J62_RS03445	758966..759223	-	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
A6J62_RS03455	759425..759958	-	534	WP_000118351.1	GNAT family N-acetyltransferase	Toxin
A6J62_RS19635	759955..760380	-	426	WP_001882332.1	DUF1778 domain-containing protein	Antitoxin
A6J62_RS03465	760447..760818	-	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
A6J62_RS03470	760959..761246	-	288	WP_000426470.1	hypothetical protein	-
A6J62_RS03475	761398..762066	-	669	WP_000043871.1	hypothetical protein	-
A6J62_RS03480	762297..762569	+	273	WP_000246253.1	DUF1778 domain-containing protein	-
A6J62_RS03485	762566..763063	+	498	WP_000982260.1	GNAT family N-acetyltransferase	-
A6J62_RS03495	763265..763417	-	153	WP_001884520.1	DUF645 family protein	-
A6J62_RS03500	763739..764194	-	456	WP_001245327.1	GNAT family N-acetyltransferase	-
A6J62_RS03505	764322..764609	-	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
A6J62_RS03510	764599..764850	-	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	749087..881909	132822
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0	inside	Integron	-	-	749463..875103	125640
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0	flank	IS/Tn	-	-	756078..756515	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (57-133)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 20171.46 Da
• Isoelectric Point: 9.1432

>T74618 WP_000118351.1 NZ_CP020407:c759958-759425 [Vibrio cholerae]
VSWGKEFVELSKSKHDRDSFDCGEQELNTFIKTQAAKHMQAGISRTMVLPSAHPLLNQKFAICAFYSVAPSSISRETLPA
QLAKKLPRYPIPVFLLAQLAVHKEFHGSGLGKVSLIRALKYLWEVNHHMRAYAIVVDCLTDSAQAFYTKFGFEVLCEHNE
RIRMFLPMKTVEQLFNQ

Nucleotide

• Length: 534 bp

>T74618 NZ_CP020407:c759958-759425 [Vibrio cholerae]
GTGAGTTGGGGCAAAGAGTTTGTAGAACTTAGTAAATCAAAGCATGACCGCGACTCATTTGACTGTGGTGAGCAAGAGCT
AAACACATTTATCAAAACTCAAGCTGCAAAGCACATGCAAGCAGGTATTAGCCGAACAATGGTTTTACCTAGTGCTCACC
CACTGTTAAATCAAAAATTCGCTATTTGTGCCTTTTATAGTGTTGCACCGAGCTCAATCAGCCGAGAAACATTACCGGCA
CAATTAGCAAAGAAACTACCGAGGTATCCAATCCCTGTTTTTCTGTTGGCTCAGTTAGCTGTGCACAAAGAATTTCACGG
TTCTGGACTTGGAAAGGTCAGTCTAATCCGTGCGCTGAAGTACTTGTGGGAAGTGAATCATCACATGAGAGCATACGCAA
TAGTTGTGGATTGTCTGACAGATTCCGCTCAAGCGTTCTATACGAAGTTCGGCTTTGAAGTATTGTGCGAACATAATGAG
CGTATTCGTATGTTCTTACCGATGAAAACGGTAGAACAACTTTTTAATCAATAG

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15714.41 Da
• Isoelectric Point: 7.6659

>AT74618 WP_001882332.1 NZ_CP020407:c760380-759955 [Vibrio cholerae]
MLCLSLVVMRCQPLRRALYSPSPRKLSVRIECALSLAYNRSTDGMYPYAGGVMATARLDIRLDEEIKAKAEKASALLGLK
SLTEYVVRLMDEDATHVIEEHESIMVKDSVFDEFMAACNKVKAPNQALLEAVKFTEKSGIK

Nucleotide

• Length: 426 bp

>AT74618 NZ_CP020407:c760380-759955 [Vibrio cholerae]
TTGTTGTGTTTGAGTTTAGTGGTCATGCGTTGTCAGCCCCTTAGACGGGCGTTATATTCTCCCTCGCCCCGTAAACTTTC
CGTACGAATTGAATGTGCTTTAAGTTTGGCCTATAATCGCTCAACTGACGGAATGTATCCGTACGCAGGAGGGGTTATGG
CAACGGCACGTTTAGATATCCGCCTTGATGAAGAGATCAAGGCAAAAGCTGAAAAGGCATCGGCTTTACTTGGTTTAAAA
AGTCTCACTGAATATGTGGTGAGACTTATGGACGAAGATGCGACTCATGTTATTGAGGAGCATGAAAGTATTATGGTTAA
AGACAGTGTTTTTGACGAGTTTATGGCTGCGTGTAATAAAGTAAAAGCACCGAATCAAGCTTTGCTTGAAGCTGTTAAAT
TTACAGAGAAGAGCGGTATCAAGTGA

Structures

Toxin

• AlphaFold DB: Q9KM94