145871

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 369326..369941 Replicon chromosome
Accession NZ_CP047302
Organism Vibrio cholerae O1 biovar El Tor strain DRC-193A

Toxin (Protein)


Gene name higB Uniprot ID Q9KMG5
Locus tag GTF75_RS15735 Protein ID WP_001162670.1
Coordinates 369326..369613 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag GTF75_RS15740 Protein ID WP_001232701.1
Coordinates 369624..369941 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTF75_RS15695 364962..365057 + 96 WP_001907607.1 DUF645 family protein -
GTF75_RS15700 365251..365568 - 318 WP_001180243.1 type II toxin-antitoxin system RelE/ParE family toxin -
GTF75_RS15705 365587..365829 - 243 WP_000557292.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
GTF75_RS15710 366071..366580 + 510 WP_000405987.1 GNAT family N-acetyltransferase -
GTF75_RS15715 366637..367290 + 654 WP_000226874.1 hypothetical protein -
GTF75_RS15720 367600..367818 + 219 WP_001917086.1 DUF3709 domain-containing protein -
GTF75_RS15725 368221..368454 + 234 WP_032482776.1 DUF3709 domain-containing protein -
GTF75_RS15730 368879..369059 + 181 Protein_365 DUF645 family protein -
GTF75_RS15735 369326..369613 + 288 WP_001162670.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GTF75_RS15740 369624..369941 + 318 WP_001232701.1 HigA family addiction module antidote protein Antitoxin
GTF75_RS15745 369938..370048 + 111 WP_134814058.1 DUF3265 domain-containing protein -
GTF75_RS15750 370225..370350 + 126 WP_001944767.1 DUF645 family protein -
GTF75_RS15755 370366..370404 + 39 WP_106019119.1 hypothetical protein -
GTF75_RS15760 370617..371621 + 1005 WP_000964925.1 LD-carboxypeptidase -
GTF75_RS15765 371774..372220 + 447 WP_000128977.1 hypothetical protein -
GTF75_RS15770 372340..373269 + 930 WP_000335802.1 hypothetical protein -
GTF75_RS15775 373266..373376 + 111 WP_071917832.1 DUF3265 domain-containing protein -
GTF75_RS15780 373404..373880 + 477 WP_001047180.1 GNAT family N-acetyltransferase -
GTF75_RS15785 374017..374532 + 516 WP_001201528.1 lipocalin family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 305935..411136 105201
inside Integron - - 311015..410760 99745


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(2-95)

Antitoxin

Cro/C1-type helix-turn-helix domain

(12-69)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11349.95 Da        Isoelectric Point: 6.7187

>T145871 WP_001162670.1 NZ_CP047302:369326-369613 [Vibrio cholerae O1 biovar El Tor]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Download         Length: 288 bp

>T145871 NZ_CP062705:2364673-2364776 [Escherichia coli O157:H7]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11705.63 Da        Isoelectric Point: 10.4711

>AT145871 WP_001232701.1 NZ_CP047302:369624-369941 [Vibrio cholerae O1 biovar El Tor]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Download         Length: 318 bp

>AT145871 NZ_CP062705:c2364814-2364543 [Escherichia coli O157:H7]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F2HI36

Antitoxin

Source ID Structure

References