Detailed information of TA system TA113801

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-GNAT
• Location: 143341..144434
• Replicon: chromosome
• Accession: NZ_CP033513
• Organism: Vibrio cholerae strain E4

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9KMA2
• Locus tag: EEL44_RS00735
• Protein ID: WP_000351248.1
• Coordinates: 143341..143742 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: EEL44_RS00740
• Protein ID: WP_001047169.1
• Coordinates: 143742..144434 (-)
• Length: 231 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EEL44_RS00680	138635..138922	-	288	WP_000426470.1	hypothetical protein	-
EEL44_RS00685	139074..139742	-	669	WP_000043871.1	hypothetical protein	-
EEL44_RS00690	139973..140245	+	273	WP_000246253.1	DUF1778 domain-containing protein	-
EEL44_RS00695	140242..140739	+	498	WP_000982260.1	GNAT family N-acetyltransferase	-
EEL44_RS00705	140941..141093	-	153	WP_001884520.1	DUF645 family protein	-
EEL44_RS00710	141415..141870	-	456	WP_001245327.1	GNAT family N-acetyltransferase	-
EEL44_RS00715	141998..142285	-	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EEL44_RS00720	142275..142526	-	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
EEL44_RS00725	142740..143153	-	414	WP_000049417.1	VOC family protein	-
EEL44_RS00730	143228..143371	-	144	WP_071908341.1	DUF3265 domain-containing protein	-
EEL44_RS00735	143341..143742	-	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
EEL44_RS00740	143742..144434	-	693	WP_001047169.1	GNAT family N-acetyltransferase	Antitoxin
EEL44_RS00745	144571..144877	-	307	Protein_138	CatB-related O-acetyltransferase	-
EEL44_RS00750	144945..145925	+	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
EEL44_RS20945	146050..146205	-	156	WP_000751734.1	hypothetical protein	-
EEL44_RS00755	146373..146807	-	435	WP_000256036.1	GNAT family N-acetyltransferase	-
EEL44_RS00760	146944..147258	-	315	WP_000071008.1	DNA-binding transcriptional regulator	-
EEL44_RS00765	147245..147577	-	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EEL44_RS00775	147918..148145	-	228	WP_001894457.1	DUF1289 domain-containing protein	-
EEL44_RS00780	148094..148207	-	114	WP_001889158.1	hypothetical protein	-
EEL44_RS00795	148373..148654	-	282	WP_001894453.1	DUF1289 domain-containing protein	-
EEL44_RS21135	148603..148717	-	115	Protein_147	acetyltransferase	-
EEL44_RS00800	148774..149151	-	378	WP_000411109.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	126763..233717	106954
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	-	-	127139..226875	99736
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	144945..145925	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-132)

Antitoxin

• GNAT domain (36-153)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14608.71 Da
• Isoelectric Point: 4.0128

>T113801 WP_000351248.1 NZ_CP033513:c143742-143341 [Vibrio cholerae]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Nucleotide

• Length: 402 bp

>T113801 NZ_CP033513:c143742-143341 [Vibrio cholerae]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin

Protein

• Length: 231 a.a.
• Molecular weight: 26352.73 Da
• Isoelectric Point: 8.5633

>AT113801 WP_001047169.1 NZ_CP033513:c144434-143742 [Vibrio cholerae]
MNLEEFQESDFDLLIKWIDSDELNYLWGCPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 693 bp

>AT113801 NZ_CP033513:c144434-143742 [Vibrio cholerae]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTTGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTATGGTTGTTGTGATTGAGTTTGGCGGTATGCGTTGTCAGCCCCTTAGGCGGGCGTTATGTTTTTTATCACGTTTT
GGGGCTATAATCGGCCTAACCTTTATTGATAGTAGGTGTGATATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGT
TGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGACTGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTA
AACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGGCTGATATGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q0PN34