64441

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 438819..439347 Replicon chromosome
Accession NZ_CP016325
Organism Vibrio cholerae 2740-80

Toxin (Protein)


Gene name relE Uniprot ID A0A0X1KZS6
Locus tag BBB50_RS16175 Protein ID WP_000221354.1
Coordinates 439060..439347 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0X1KZS8
Locus tag BBB50_RS16170 Protein ID WP_001250179.1
Coordinates 438819..439070 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BBB50_RS16130 434087..434401 + 315 WP_000071008.1 DNA-binding transcriptional regulator -
BBB50_RS16135 434538..434972 + 435 WP_000256036.1 GNAT family N-acetyltransferase -
BBB50_RS16140 435140..435295 + 156 WP_000751734.1 hypothetical protein -
BBB50_RS16145 435420..436400 - 981 WP_000019370.1 IS5-like element ISVch5 family transposase -
BBB50_RS16150 436468..436774 + 307 Protein_465 CatB-related O-acetyltransferase -
BBB50_RS16155 436911..437603 + 693 WP_001047169.1 GNAT family N-acetyltransferase -
BBB50_RS16160 437603..438004 + 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin -
BBB50_RS20340 437974..438117 + 144 WP_071908341.1 DUF3265 domain-containing protein -
BBB50_RS16165 438192..438605 + 414 WP_000049417.1 VOC family protein -
BBB50_RS16170 438819..439070 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
BBB50_RS16175 439060..439347 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BBB50_RS16180 439475..439930 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
BBB50_RS20000 440252..440404 + 153 WP_001884520.1 DUF645 family protein -
BBB50_RS16185 440606..441103 - 498 WP_000982260.1 GNAT family N-acetyltransferase -
BBB50_RS16190 441100..441372 - 273 WP_000246253.1 DUF1778 domain-containing protein -
BBB50_RS16195 441603..442271 + 669 WP_000043871.1 hypothetical protein -
BBB50_RS16200 442423..442710 + 288 WP_000426470.1 hypothetical protein -
BBB50_RS16205 442851..443222 + 372 WP_001164080.1 nucleotide pyrophosphohydrolase -
BBB50_RS20545 443289..443714 + 426 WP_001882332.1 DUF1778 domain-containing protein -
BBB50_RS16215 443711..444244 + 534 WP_000118351.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 304243..451810 147567
- inside Integron - - 309323..451434 142111
flank IS/Tn - - 435420..436400 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(11-71)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 10991.96 Da        Isoelectric Point: 10.4934

>T64441 WP_000221354.1 NZ_CP016325:439060-439347 [Vibrio cholerae 2740-80]
MTYKLKFLPAAQKEWSKLAPTIQSQFKKKLKERLENPHVPSAKLRGYDAVYKIKLRTAGYRLAYEVIDDEIVVYVLAVGK
RDKDAVYKKLASRFG

Download         Length: 288 bp

>T64441 NZ_CP016325:439060-439347 [Vibrio cholerae 2740-80]
ATGACCTATAAGTTAAAGTTTCTGCCGGCTGCGCAAAAGGAATGGAGTAAGTTAGCTCCGACAATTCAGAGTCAGTTCAA
GAAGAAGTTAAAGGAACGATTAGAAAATCCGCACGTCCCTTCGGCTAAGCTTCGAGGATACGACGCTGTTTACAAGATTA
AACTTCGTACGGCGGGTTATCGTTTAGCTTATGAGGTTATTGACGATGAGATAGTTGTCTATGTCCTTGCTGTCGGTAAA
CGTGACAAAGATGCTGTCTATAAAAAACTGGCTTCACGCTTCGGTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9136.29 Da        Isoelectric Point: 4.0197

>AT64441 WP_001250179.1 NZ_CP016325:438819-439070 [Vibrio cholerae 2740-80]
MRQVLANCSASISELKKNPTALLNEADGSAIAILNHNKPAAYLVPAETYEYLIDMLDDYELSQIVDSRRADLAQAVEVNI
DDL

Download         Length: 252 bp

>AT64441 NZ_CP016325:438819-439070 [Vibrio cholerae 2740-80]
ATGAGACAAGTTTTAGCGAATTGCTCTGCAAGTATTTCAGAGTTAAAGAAGAACCCAACAGCTTTGTTGAATGAGGCTGA
TGGGTCTGCAATTGCAATTTTAAACCACAACAAGCCAGCGGCTTACCTTGTGCCAGCGGAAACGTATGAGTATCTCATCG
ATATGCTCGATGATTATGAGCTTTCCCAAATTGTCGATAGTCGCCGAGCTGACTTAGCACAAGCTGTGGAAGTAAATATT
GATGACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0X1KZS6

Antitoxin

Source ID Structure
AlphaFold DB A0A0X1KZS8

References