230650

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 921538..922304 Replicon chromosome
Accession NZ_CP090400
Organism Vibrio cholerae strain PanChol

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag LZM82_RS18075 Protein ID WP_000982260.1
Coordinates 921807..922304 (+) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9K2J6
Locus tag LZM82_RS18070 Protein ID WP_000246253.1
Coordinates 921538..921810 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZM82_RS18020 (LZM82_18020) 916624..917010 - 387 WP_000703163.1 VOC family protein -
LZM82_RS18025 (LZM82_18025) 917000..917101 - 102 WP_001921603.1 hypothetical protein -
LZM82_RS18030 (LZM82_18030) 917301..917399 - 99 WP_223225486.1 DUF3709 domain-containing protein -
LZM82_RS18035 (LZM82_18035) 917759..918037 - 279 WP_001921601.1 DUF3709 domain-containing protein -
LZM82_RS18040 (LZM82_18040) 918155..918272 - 118 Protein_876 DUF3265 domain-containing protein -
LZM82_RS18045 (LZM82_18045) 918323..918601 + 279 WP_001258569.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
LZM82_RS18050 (LZM82_18050) 918594..918863 + 270 WP_000277238.1 type II toxin-antitoxin system YafQ family toxin -
LZM82_RS18055 (LZM82_18055) 919050..920261 - 1212 WP_001122813.1 IS256 family transposase -
LZM82_RS18060 (LZM82_18060) 920324..920770 - 447 WP_000006157.1 hypothetical protein -
LZM82_RS18065 (LZM82_18065) 921020..921145 - 126 WP_001900195.1 DUF645 family protein -
LZM82_RS18070 (LZM82_18070) 921538..921810 + 273 WP_000246253.1 DUF1778 domain-containing protein Antitoxin
LZM82_RS18075 (LZM82_18075) 921807..922304 + 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
LZM82_RS18080 (LZM82_18080) 922321..922389 - 69 Protein_884 acetyltransferase -
LZM82_RS18085 (LZM82_18085) 922454..922915 - 462 WP_001903091.1 lipocalin family protein -
LZM82_RS18090 (LZM82_18090) 923106..923642 - 537 WP_000469482.1 GNAT family protein -
LZM82_RS18095 (LZM82_18095) 923900..924121 - 222 WP_032467793.1 DUF1289 domain-containing protein -
LZM82_RS18100 (LZM82_18100) 924130..924243 - 114 WP_001900214.1 hypothetical protein -
LZM82_RS18105 (LZM82_18105) 924300..924686 - 387 WP_001015867.1 NUDIX hydrolase -
LZM82_RS18110 (LZM82_18110) 924930..925172 + 243 WP_000557292.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LZM82_RS18115 (LZM82_18115) 925191..925508 + 318 WP_001180243.1 type II toxin-antitoxin system RelE/ParE family toxin -
LZM82_RS18120 (LZM82_18120) 925662..926000 - 339 WP_000713853.1 hypothetical protein -
LZM82_RS18125 (LZM82_18125) 926158..926862 - 705 WP_000087610.1 HNH endonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 835643..943910 108267
inside Integron - - 836019..937068 101049
- flank IS/Tn - - 919050..920261 1211


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T230650 WP_000982260.1 NZ_CP090400:921807-922304 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T230650 NZ_CP125279:485298-485648 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9683.22 Da        Isoelectric Point: 6.2988

>AT230650 WP_000246253.1 NZ_CP090400:921538-921810 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT230650 NZ_CP125279:485011-485292 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
AlphaFold DB A0A0F2IC79

References