Detailed information of TA system TA023785

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-GNAT
• Location: 967062..968155
• Replicon: chromosome
• Accession: NC_012667
• Organism: Vibrio cholerae MJ-1236

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9KMA2
• Locus tag: VCD_RS18980
• Protein ID: WP_000351248.1
• Coordinates: 967062..967463 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: VCD_RS18985
• Protein ID: WP_001047169.1
• Coordinates: 967463..968155 (-)
• Length: 231 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VCD_RS18935	962356..962643	-	288	WP_000426470.1	hypothetical protein	-
VCD_RS18940	962795..963463	-	669	WP_000043871.1	hypothetical protein	-
VCD_RS18945	963694..963966	+	273	WP_000246253.1	DUF1778 domain-containing protein	-
VCD_RS18950	963963..964460	+	498	WP_000982260.1	GNAT family N-acetyltransferase	-
VCD_RS18955	964662..964814	-	153	WP_001884520.1	DUF645 family protein	-
VCD_RS18960	965136..965591	-	456	WP_001245327.1	GNAT family N-acetyltransferase	-
VCD_RS18965	965719..966006	-	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
VCD_RS18970	965996..966247	-	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
VCD_RS18975	966461..966874	-	414	WP_000049417.1	VOC family protein	-
VCD_RS20710	966949..967092	-	144	WP_071908341.1	DUF3265 domain-containing protein	-
VCD_RS18980	967062..967463	-	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
VCD_RS18985	967463..968155	-	693	WP_001047169.1	GNAT family N-acetyltransferase	Antitoxin
VCD_RS18990	968292..968598	-	307	Protein_849	CatB-related O-acetyltransferase	-
VCD_RS18995	968666..969646	+	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
VCD_RS19000	969771..969926	-	156	WP_000751734.1	hypothetical protein	-
VCD_RS19005	970094..970528	-	435	WP_000256036.1	GNAT family N-acetyltransferase	-
VCD_RS19010	970665..970979	-	315	WP_000071008.1	DNA-binding transcriptional regulator	-
VCD_RS19015	970966..971298	-	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	-
VCD_RS20360	971639..971866	-	228	WP_001894457.1	DUF1289 domain-containing protein	-
VCD_RS20365	971815..971928	-	114	WP_001889158.1	hypothetical protein	-
VCD_RS20380	972094..972375	-	282	WP_001894453.1	DUF1289 domain-containing protein	-
VCD_RS21065	972324..972438	-	115	Protein_858	acetyltransferase	-
VCD_RS19025	972495..972872	-	378	WP_000411109.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	932253..1057432	125179
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.006	inside	Integron	-	-	958376..1050596	92220
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	968666..969646	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-132)

Antitoxin

• GNAT domain (36-153)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14608.71 Da
• Isoelectric Point: 4.0128

>T23785 WP_000351248.1 NC_012667:c967463-967062 [Vibrio cholerae MJ-1236]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Nucleotide

• Length: 402 bp

>T23785 NC_012667:c967463-967062 [Vibrio cholerae MJ-1236]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin

Protein

• Length: 231 a.a.
• Molecular weight: 26352.73 Da
• Isoelectric Point: 8.5633

>AT23785 WP_001047169.1 NC_012667:c968155-967463 [Vibrio cholerae MJ-1236]
MNLEEFQESDFDLLIKWIDSDELNYLWGCPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 693 bp

>AT23785 NC_012667:c968155-967463 [Vibrio cholerae MJ-1236]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTTGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTATGGTTGTTGTGATTGAGTTTGGCGGTATGCGTTGTCAGCCCCTTAGGCGGGCGTTATGTTTTTTATCACGTTTT
GGGGCTATAATCGGCCTAACCTTTATTGATAGTAGGTGTGATATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGT
TGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGACTGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTA
AACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGGCTGATATGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q0PN34