Detailed information of TA system TA045818

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DhiAT/-
• Location: 406416..407200
• Replicon: chromosome
• Accession: NZ_CP006948
• Organism: Vibrio cholerae O1 str. KW3

Toxin (Protein)

• Gene name: dhiT
• Uniprot ID: A0A086SLD6
• Locus tag: N900_RS16125
• Protein ID: WP_001114075.1
• Coordinates: 406416..406685 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: dhiA
• Uniprot ID: Q9KM84
• Locus tag: N900_RS16130
• Protein ID: WP_000921691.1
• Coordinates: 406679..407200 (+)
• Length: 174 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N900_RS16090	401783..402699	+	917	Protein_421	alpha/beta hydrolase	-
N900_RS16095	402856..403245	+	390	WP_001081302.1	hypothetical protein	-
N900_RS16100	403381..403590	+	210	Protein_423	GNAT family N-acetyltransferase	-
N900_RS16105	403709..404146	+	438	WP_000503169.1	IS200/IS605-like element IS1004 family transposase	-
N900_RS16110	404210..404428	+	219	WP_071908318.1	GNAT family N-acetyltransferase	-
N900_RS16115	404603..405475	+	873	WP_000254716.1	DUF3800 domain-containing protein	-
N900_RS16120	405625..406224	+	600	WP_001891245.1	glutathione S-transferase N-terminal domain-containing protein	-
N900_RS20070	406281..406385	+	105	WP_099607150.1	acetyltransferase	-
N900_RS16125	406416..406685	+	270	WP_001114075.1	DUF4160 domain-containing protein	Toxin
N900_RS16130	406679..407200	+	522	WP_000921691.1	DUF2442 domain-containing protein	Antitoxin
N900_RS20075	407499..407624	+	126	WP_001905700.1	DUF645 family protein	-
N900_RS16135	407875..408270	+	396	WP_001000867.1	hypothetical protein	-
N900_RS16140	408409..408687	-	279	WP_000578476.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
N900_RS16145	408684..408968	-	285	WP_000381183.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
N900_RS16150	409161..409676	+	516	WP_000343779.1	GNAT family N-acetyltransferase	-
N900_RS16155	409860..410273	+	414	WP_000049420.1	VOC family protein	-
N900_RS16165	410828..412004	+	1177	Protein_437	IS3-like element ISVch4 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304645..411088	106443
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	-	-	309725..410760	101035
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0	flank	IS/Tn	-	-	403709..404146	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF4160 (9-74)

Antitoxin

• Protein of unknown function DUF2442 (4-59)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10310.94 Da
• Isoelectric Point: 6.6303

>T45818 WP_001114075.1 NZ_CP006948:406416-406685 [Vibrio cholerae O1 str. KW3]
MPEIDALLGLSFCLYFFDNKQHKLPHIHVKYGSYELIIAIETGECLEGYLPNKQRKRAENHILEHREQLMVMWNKAVNGE
NPGKLGDLC

Nucleotide

• Length: 270 bp

>T45818 NZ_CP006948:406416-406685 [Vibrio cholerae O1 str. KW3]
ATGCCAGAGATTGATGCCCTATTAGGTCTTTCATTTTGCTTGTACTTCTTTGATAACAAGCAGCATAAATTACCTCATAT
TCACGTTAAGTACGGTAGTTACGAGCTAATTATTGCTATTGAAACAGGTGAGTGTTTAGAGGGTTACTTACCCAATAAGC
AACGAAAACGTGCTGAAAACCATATTCTTGAACATCGAGAGCAATTGATGGTGATGTGGAATAAAGCGGTGAATGGTGAA
AATCCAGGTAAGTTAGGTGATTTATGTTGA

Antitoxin

Protein

• Length: 174 a.a.
• Molecular weight: 19409.01 Da
• Isoelectric Point: 6.7342

>AT45818 WP_000921691.1 NZ_CP006948:406679-407200 [Vibrio cholerae O1 str. KW3]
MLKVIDVDFVSDHTLELTFNDGYQGYADLSVYFKKAPFSEIKDFKRFSLTRDGSLNWDGNELTAATLRDITKGSQKSVEL
SFNVQEMEAVIKQASWESMMEGRPDILQAAIRSYVEQFGHGQVIAKAGIKSRTSAYRSLKPETTPNFGTLVQLGHAVIEL
AKDRTVANKEPRI

Nucleotide

• Length: 522 bp

>AT45818 NZ_CP006948:406679-407200 [Vibrio cholerae O1 str. KW3]
ATGTTGAAAGTCATAGATGTTGATTTTGTCTCAGACCATACATTAGAACTGACGTTCAATGATGGTTACCAAGGCTATGC
CGACTTGTCGGTATACTTTAAAAAAGCACCGTTTTCAGAAATTAAGGACTTTAAACGCTTTTCCTTGACTCGTGATGGCT
CGTTAAATTGGGATGGTAATGAGTTAACGGCCGCGACACTCCGTGACATAACCAAGGGCTCACAAAAGTCGGTGGAGTTA
AGTTTTAATGTTCAAGAAATGGAAGCCGTCATCAAACAGGCCTCATGGGAATCGATGATGGAGGGGCGTCCCGACATCTT
GCAAGCGGCTATTCGTTCTTACGTAGAGCAGTTTGGTCATGGTCAAGTTATTGCAAAGGCGGGTATAAAAAGTCGTACGA
GTGCTTATCGCTCCTTGAAGCCAGAAACAACGCCAAACTTTGGCACACTAGTTCAGTTAGGGCACGCCGTCATCGAACTA
GCGAAAGATAGAACAGTGGCAAACAAGGAACCACGCATATAA

Structures

Toxin

• AlphaFold DB: A0A086SLD6

Antitoxin

• AlphaFold DB: Q9KM84