230527

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /DUF1778(antitoxin)
Location 388755..389554 Replicon chromosome
Accession NZ_CP090379
Organism Vibrio cholerae strain BY369

Toxin (Protein)


Gene name - Uniprot ID Q9KM94
Locus tag LVJ30_RS15940 Protein ID WP_000118351.1
Coordinates 389021..389554 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A0K9UER4
Locus tag LVJ30_RS15935 Protein ID WP_000179600.1
Coordinates 388755..389024 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ30_RS15875 (LVJ30_15875) 384129..384380 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LVJ30_RS15880 (LVJ30_15880) 384370..384657 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin -
LVJ30_RS15885 (LVJ30_15885) 384785..385240 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
LVJ30_RS15890 (LVJ30_15890) 385297..385419 + 123 Protein_411 acetyltransferase -
LVJ30_RS15895 (LVJ30_15895) 385590..385714 + 125 Protein_412 DUF645 family protein -
LVJ30_RS15900 (LVJ30_15900) 385831..385899 + 69 Protein_413 acetyltransferase -
LVJ30_RS15905 (LVJ30_15905) 385916..386413 - 498 WP_000982260.1 GNAT family N-acetyltransferase -
LVJ30_RS15910 (LVJ30_15910) 386410..386682 - 273 WP_000246253.1 DUF1778 domain-containing protein -
LVJ30_RS15915 (LVJ30_15915) 386834..386896 + 63 Protein_416 acetyltransferase -
LVJ30_RS15920 (LVJ30_15920) 386913..387581 + 669 WP_000043871.1 hypothetical protein -
LVJ30_RS15925 (LVJ30_15925) 387733..388020 + 288 WP_000426470.1 hypothetical protein -
LVJ30_RS15930 (LVJ30_15930) 388161..388532 + 372 WP_001164080.1 MazG nucleotide pyrophosphohydrolase domain-containing protein -
LVJ30_RS15935 (LVJ30_15935) 388755..389024 + 270 WP_000179600.1 DUF1778 domain-containing protein Antitoxin
LVJ30_RS15940 (LVJ30_15940) 389021..389554 + 534 WP_000118351.1 GNAT family N-acetyltransferase Toxin
LVJ30_RS15945 (LVJ30_15945) 389564..389674 + 111 WP_086010065.1 DUF3265 domain-containing protein -
LVJ30_RS15950 (LVJ30_15950) 389756..390013 + 258 WP_000861987.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LVJ30_RS15955 (LVJ30_15955) 390001..390303 + 303 WP_000229317.1 type II toxin-antitoxin system RelE/ParE family toxin -
LVJ30_RS15960 (LVJ30_15960) 390537..391454 + 918 WP_000186333.1 alpha/beta hydrolase -
LVJ30_RS15965 (LVJ30_15965) 391611..392000 + 390 WP_001081302.1 hypothetical protein -
LVJ30_RS15970 (LVJ30_15970) 392136..392345 + 210 Protein_427 GNAT family N-acetyltransferase -
LVJ30_RS15975 (LVJ30_15975) 392464..392901 + 438 WP_000503169.1 IS200/IS605-like element IS1004 family transposase -
LVJ30_RS15980 (LVJ30_15980) 392962..393183 + 222 Protein_429 GNAT family N-acetyltransferase -
LVJ30_RS15985 (LVJ30_15985) 393358..394230 + 873 WP_000254716.1 DUF3800 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 304645..399892 95247
inside Integron - - 309725..399516 89791
flank IS/Tn - - 392464..392901 437


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(97-153)

Antitoxin

Vibrio phage ICP1, Orf50

(5-81)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 20171.46 Da        Isoelectric Point: 9.1432

>T230527 WP_000118351.1 NZ_CP090379:389021-389554 [Vibrio cholerae]
VSWGKEFVELSKSKHDRDSFDCGEQELNTFIKTQAAKHMQAGISRTMVLPSAHPLLNQKFAICAFYSVAPSSISRETLPA
QLAKKLPRYPIPVFLLAQLAVHKEFHGSGLGKVSLIRALKYLWEVNHHMRAYAIVVDCLTDSAQAFYTKFGFEVLCEHNE
RIRMFLPMKTVEQLFNQ

Download         Length: 534 bp

>T230527 NZ_CP124918:2463940-2464284 [Enterococcus faecalis]
ATGAAAAGTATCAAAGAGTTGGTAGAAGAATATAATGTGGAGTTAGTTTTTACTACTTTGAACAAACGCGCATGTTTCGA
CCCTACCTACGGTATCATATTTGTAAATCAAAATTTAACACCATCAGAACAAGAAGAAGCAATATATCACGAGTTAAAGC
ATGTAAAAGACCATGTGGATATAATGGAATTGTATAAAATTCCTGTTTTTCGTTCTAAGATGGAATCCGAAGCAGAACAA
TATATGTTTAGAAGCTTAATCGAAAAATATGAAGGACAATACAATTACTCAAATGTTATAGCTCATTACAACTTAAAAAT
GGGACAAGAAGTTTATTTGAAATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9924.49 Da        Isoelectric Point: 4.9107

>AT230527 WP_000179600.1 NZ_CP090379:388755-389024 [Vibrio cholerae]
MATARLDIRLDEEIKAKAEKASALLGLKSLTEYVVRLMDEDATHVIEEHESIMVKDSVFDEFMAACNKVKAPNQALLEAV
KFTEKSGIK

Download         Length: 270 bp

>AT230527 NZ_CP124918:2463589-2463921 [Enterococcus faecalis]
ATGAATACTTATGAAATAATAAAAGAGTTGACAAAAAGGAAGAAAATGTCTATTCGACAATTAGAAATTACTCTAGGTTA
CTCAAATGGATATTTTAGCAAGTGGAAAAAAGTTTCTCCAAACTCAGAAGGCCTACAAAAAGTTGCGGACTACTTCAATG
TATCGGTAGATTATCTATTGGGAAGAACTGATAATACTAAAGCAACTGATGAAAAAAATTCTGATGATTTAGATGATGTA
CTGGATAACGTCATGAGTTTTGACGGTGAACCGCTTGATGATCATGACAGAGAAGTTATTCGTGCATATTTAAAGGGTAG
ATTCGGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q9KM94

Antitoxin

Source ID Structure
AlphaFold DB A0A0K9UER4

References