Detailed information of TA system TA023782

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 960822..961777
• Replicon: chromosome
• Accession: NC_012667
• Organism: Vibrio cholerae MJ-1236

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KM94
• Locus tag: VCD_RS18920
• Protein ID: WP_000118351.1
• Coordinates: 960822..961355 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: VCD_RS21060
• Protein ID: WP_001882332.1
• Coordinates: 961352..961777 (-)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VCD_RS18890	955974..957722	+	1749	WP_000063385.1	hypothetical protein	-
VCD_RS18895	957722..958180	+	459	WP_001176612.1	hypothetical protein	-
VCD_RS18900	958376..958765	-	390	WP_001081302.1	hypothetical protein	-
VCD_RS18905	958922..959839	-	918	WP_000186333.1	alpha/beta hydrolase	-
VCD_RS18910	960073..960375	-	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
VCD_RS18915	960363..960620	-	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
VCD_RS18920	960822..961355	-	534	WP_000118351.1	GNAT family N-acetyltransferase	Toxin
VCD_RS21060	961352..961777	-	426	WP_001882332.1	DUF1778 domain-containing protein	Antitoxin
VCD_RS18930	961844..962215	-	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
VCD_RS18935	962356..962643	-	288	WP_000426470.1	hypothetical protein	-
VCD_RS18940	962795..963463	-	669	WP_000043871.1	hypothetical protein	-
VCD_RS18945	963694..963966	+	273	WP_000246253.1	DUF1778 domain-containing protein	-
VCD_RS18950	963963..964460	+	498	WP_000982260.1	GNAT family N-acetyltransferase	-
VCD_RS18955	964662..964814	-	153	WP_001884520.1	DUF645 family protein	-
VCD_RS18960	965136..965591	-	456	WP_001245327.1	GNAT family N-acetyltransferase	-
VCD_RS18965	965719..966006	-	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
VCD_RS18970	965996..966247	-	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	932253..1057432	125179
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.006	inside	Integron	-	-	958376..1050596	92220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (57-133)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 20171.46 Da
• Isoelectric Point: 9.1432

>T23782 WP_000118351.1 NC_012667:c961355-960822 [Vibrio cholerae MJ-1236]
VSWGKEFVELSKSKHDRDSFDCGEQELNTFIKTQAAKHMQAGISRTMVLPSAHPLLNQKFAICAFYSVAPSSISRETLPA
QLAKKLPRYPIPVFLLAQLAVHKEFHGSGLGKVSLIRALKYLWEVNHHMRAYAIVVDCLTDSAQAFYTKFGFEVLCEHNE
RIRMFLPMKTVEQLFNQ

Nucleotide

• Length: 534 bp

>T23782 NC_012667:c961355-960822 [Vibrio cholerae MJ-1236]
GTGAGTTGGGGCAAAGAGTTTGTAGAACTTAGTAAATCAAAGCATGACCGCGACTCATTTGACTGTGGTGAGCAAGAGCT
AAACACATTTATCAAAACTCAAGCTGCAAAGCACATGCAAGCAGGTATTAGCCGAACAATGGTTTTACCTAGTGCTCACC
CACTGTTAAATCAAAAATTCGCTATTTGTGCCTTTTATAGTGTTGCACCGAGCTCAATCAGCCGAGAAACATTACCGGCA
CAATTAGCAAAGAAACTACCGAGGTATCCAATCCCTGTTTTTCTGTTGGCTCAGTTAGCTGTGCACAAAGAATTTCACGG
TTCTGGACTTGGAAAGGTCAGTCTAATCCGTGCGCTGAAGTACTTGTGGGAAGTGAATCATCACATGAGAGCATACGCAA
TAGTTGTGGATTGTCTGACAGATTCCGCTCAAGCGTTCTATACGAAGTTCGGCTTTGAAGTATTGTGCGAACATAATGAG
CGTATTCGTATGTTCTTACCGATGAAAACGGTAGAACAACTTTTTAATCAATAG

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15714.41 Da
• Isoelectric Point: 7.6659

>AT23782 WP_001882332.1 NC_012667:c961777-961352 [Vibrio cholerae MJ-1236]
MLCLSLVVMRCQPLRRALYSPSPRKLSVRIECALSLAYNRSTDGMYPYAGGVMATARLDIRLDEEIKAKAEKASALLGLK
SLTEYVVRLMDEDATHVIEEHESIMVKDSVFDEFMAACNKVKAPNQALLEAVKFTEKSGIK

Nucleotide

• Length: 426 bp

>AT23782 NC_012667:c961777-961352 [Vibrio cholerae MJ-1236]
TTGTTGTGTTTGAGTTTAGTGGTCATGCGTTGTCAGCCCCTTAGACGGGCGTTATATTCTCCCTCGCCCCGTAAACTTTC
CGTACGAATTGAATGTGCTTTAAGTTTGGCCTATAATCGCTCAACTGACGGAATGTATCCGTACGCAGGAGGGGTTATGG
CAACGGCACGTTTAGATATCCGCCTTGATGAAGAGATCAAGGCAAAAGCTGAAAAGGCATCGGCTTTACTTGGTTTAAAA
AGTCTCACTGAATATGTGGTGAGACTTATGGACGAAGATGCGACTCATGTTATTGAGGAGCATGAAAGTATTATGGTTAA
AGACAGTGTTTTTGACGAGTTTATGGCTGCGTGTAATAAAGTAAAAGCACCGAATCAAGCTTTGCTTGAAGCTGTTAAAT
TTACAGAGAAGAGCGGTATCAAGTGA

Structures

Toxin

• AlphaFold DB: Q9KM94