58405

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 481070..481598 Replicon chromosome
Accession NZ_CP013318
Organism Vibrio cholerae strain NCTC5395

Toxin (Protein)


Gene name relE Uniprot ID A0A0X1KZS6
Locus tag ASZ85_RS17075 Protein ID WP_000221354.1
Coordinates 481311..481598 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0X1KZS8
Locus tag ASZ85_RS17070 Protein ID WP_001250179.1
Coordinates 481070..481321 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ASZ85_RS17020 476338..476652 + 315 WP_000071008.1 DNA-binding transcriptional regulator -
ASZ85_RS17025 476789..477223 + 435 WP_000256036.1 GNAT family N-acetyltransferase -
ASZ85_RS21305 477391..477546 + 156 WP_000751734.1 hypothetical protein -
ASZ85_RS17040 477671..478651 - 981 WP_000019370.1 IS5-like element ISVch5 family transposase -
ASZ85_RS17045 478719..479025 + 307 Protein_529 CatB-related O-acetyltransferase -
ASZ85_RS17050 479162..479854 + 693 WP_001047169.1 GNAT family N-acetyltransferase -
ASZ85_RS17055 479854..480255 + 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin -
ASZ85_RS21215 480225..480368 + 144 WP_071908341.1 DUF3265 domain-containing protein -
ASZ85_RS17065 480443..480856 + 414 WP_000049417.1 VOC family protein -
ASZ85_RS17070 481070..481321 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
ASZ85_RS17075 481311..481598 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ASZ85_RS17080 481726..482181 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
ASZ85_RS17090 482503..482655 + 153 WP_001884520.1 DUF645 family protein -
ASZ85_RS17095 482857..483354 - 498 WP_000982260.1 GNAT family N-acetyltransferase -
ASZ85_RS17100 483351..483623 - 273 WP_000246253.1 DUF1778 domain-containing protein -
ASZ85_RS17105 483854..484522 + 669 WP_000043871.1 hypothetical protein -
ASZ85_RS17110 484674..484961 + 288 WP_000426470.1 hypothetical protein -
ASZ85_RS17115 485102..485473 + 372 WP_001164080.1 nucleotide pyrophosphohydrolase -
ASZ85_RS17120 485540..485965 + 426 WP_001882332.1 DUF1778 domain-containing protein -
ASZ85_RS17125 485962..486495 + 534 WP_000118351.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 338285..496833 158548
inside Integron - - 343365..496457 153092
flank IS/Tn - - 477671..478651 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(11-71)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 10991.96 Da        Isoelectric Point: 10.4934

>T58405 WP_000221354.1 NZ_CP013318:481311-481598 [Vibrio cholerae]
MTYKLKFLPAAQKEWSKLAPTIQSQFKKKLKERLENPHVPSAKLRGYDAVYKIKLRTAGYRLAYEVIDDEIVVYVLAVGK
RDKDAVYKKLASRFG

Download         Length: 288 bp

>T58405 NZ_CP013318:481311-481598 [Vibrio cholerae]
ATGACCTATAAGTTAAAGTTTCTGCCGGCTGCGCAAAAGGAATGGAGTAAGTTAGCTCCGACAATTCAGAGTCAGTTCAA
GAAGAAGTTAAAGGAACGATTAGAAAATCCGCACGTCCCTTCGGCTAAGCTTCGAGGATACGACGCTGTTTACAAGATTA
AACTTCGTACGGCGGGTTATCGTTTAGCTTATGAGGTTATTGACGATGAGATAGTTGTCTATGTCCTTGCTGTCGGTAAA
CGTGACAAAGATGCTGTCTATAAAAAACTGGCTTCACGCTTCGGTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9136.29 Da        Isoelectric Point: 4.0197

>AT58405 WP_001250179.1 NZ_CP013318:481070-481321 [Vibrio cholerae]
MRQVLANCSASISELKKNPTALLNEADGSAIAILNHNKPAAYLVPAETYEYLIDMLDDYELSQIVDSRRADLAQAVEVNI
DDL

Download         Length: 252 bp

>AT58405 NZ_CP013318:481070-481321 [Vibrio cholerae]
ATGAGACAAGTTTTAGCGAATTGCTCTGCAAGTATTTCAGAGTTAAAGAAGAACCCAACAGCTTTGTTGAATGAGGCTGA
TGGGTCTGCAATTGCAATTTTAAACCACAACAAGCCAGCGGCTTACCTTGTGCCAGCGGAAACGTATGAGTATCTCATCG
ATATGCTCGATGATTATGAGCTTTCCCAAATTGTCGATAGTCGCCGAGCTGACTTAGCACAAGCTGTGGAAGTAAATATT
GATGACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0X1KZS6

Antitoxin

Source ID Structure
AlphaFold DB A0A0X1KZS8

References