Detailed information of TA system TA104257

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-GNAT
• Location: 420402..421495
• Replicon: chromosome
• Accession: NZ_CP028828
• Organism: Vibrio cholerae strain N16961

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9KMA2
• Locus tag: N16961_RS16550
• Protein ID: WP_000351248.1
• Coordinates: 421094..421495 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: N16961_RS16545
• Protein ID: WP_001047169.1
• Coordinates: 420402..421094 (+)
• Length: 231 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N16961_RS16480	415685..416062	+	378	WP_000411109.1	hypothetical protein	-
N16961_RS19945	416119..416233	+	115	Protein_439	acetyltransferase	-
N16961_RS16485	416182..416463	+	282	WP_001894453.1	DUF1289 domain-containing protein	-
N16961_RS16500	416629..416742	+	114	WP_001889158.1	hypothetical protein	-
N16961_RS16505	416691..416918	+	228	WP_001894457.1	DUF1289 domain-containing protein	-
N16961_RS16520	417259..417591	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	-
N16961_RS16525	417578..417892	+	315	WP_000071008.1	DNA-binding transcriptional regulator	-
N16961_RS16530	418029..418463	+	435	WP_000256036.1	GNAT family N-acetyltransferase	-
N16961_RS19670	418631..418786	+	156	WP_000751734.1	hypothetical protein	-
N16961_RS16535	418911..419891	-	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
N16961_RS16540	419959..420265	+	307	Protein_448	CatB-related O-acetyltransferase	-
N16961_RS16545	420402..421094	+	693	WP_001047169.1	GNAT family N-acetyltransferase	Antitoxin
N16961_RS16550	421094..421495	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
N16961_RS16555	421465..421608	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
N16961_RS16560	421683..422096	+	414	WP_000049417.1	VOC family protein	-
N16961_RS16565	422310..422561	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
N16961_RS16570	422551..422838	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
N16961_RS16575	422966..423421	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
N16961_RS16580	423743..423895	+	153	WP_001884520.1	DUF645 family protein	-
N16961_RS16590	424097..424594	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
N16961_RS16595	424591..424863	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
N16961_RS16600	425094..425762	+	669	WP_000043871.1	hypothetical protein	-
N16961_RS16605	425914..426201	+	288	WP_000426470.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	306977..438073	131096
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0	inside	Integron	-	-	312057..437697	125640
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	418911..419891	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-132)

Antitoxin

• GNAT domain (36-153)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14608.71 Da
• Isoelectric Point: 4.0128

>T104257 WP_000351248.1 NZ_CP028828:421094-421495 [Vibrio cholerae]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Nucleotide

• Length: 402 bp

>T104257 NZ_CP028828:421094-421495 [Vibrio cholerae]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin

Protein

• Length: 231 a.a.
• Molecular weight: 26352.73 Da
• Isoelectric Point: 8.5633

>AT104257 WP_001047169.1 NZ_CP028828:420402-421094 [Vibrio cholerae]
MNLEEFQESDFDLLIKWIDSDELNYLWGCPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 693 bp

>AT104257 NZ_CP028828:420402-421094 [Vibrio cholerae]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTTGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTATGGTTGTTGTGATTGAGTTTGGCGGTATGCGTTGTCAGCCCCTTAGGCGGGCGTTATGTTTTTTATCACGTTTT
GGGGCTATAATCGGCCTAACCTTTATTGATAGTAGGTGTGATATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGT
TGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGACTGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTA
AACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGGCTGATATGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q0PN34