Detailed information of TA system TA058213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 325524..326290
• Replicon: chromosome
• Accession: NZ_CP013302
• Organism: Vibrio cholerae strain C5

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A233HKB4
• Locus tag: ASZ80_RS15705
• Protein ID: WP_000982256.1
• Coordinates: 325524..326021 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: ASZ80_RS15710
• Protein ID: WP_000246254.1
• Coordinates: 326018..326290 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ80_RS15670	320924..321463	+	540	WP_053035032.1	phosphoglycerate mutase family protein	-
ASZ80_RS15675	321598..322023	+	426	WP_000693748.1	hypothetical protein	-
ASZ80_RS20930	322080..322190	+	111	WP_106746893.1	iron transporter	-
ASZ80_RS15680	322177..322536	+	360	WP_001071527.1	VOC family protein	-
ASZ80_RS21035	322678..323111	+	434	Protein_277	GNAT family N-acetyltransferase	-
ASZ80_RS15690	323248..324174	+	927	WP_000377813.1	hypothetical protein	-
ASZ80_RS20375	324497..324650	+	154	Protein_279	DUF645 family protein	-
ASZ80_RS15700	324879..325382	+	504	WP_001158950.1	DinB family protein	-
ASZ80_RS15705	325524..326021	-	498	WP_000982256.1	GNAT family N-acetyltransferase	Toxin
ASZ80_RS15710	326018..326290	-	273	WP_000246254.1	DUF1778 domain-containing protein	Antitoxin
ASZ80_RS15715	326678..326845	+	168	WP_053035038.1	DUF645 family protein	-
ASZ80_RS15720	327053..327400	+	348	WP_000933413.1	hypothetical protein	-
ASZ80_RS15730	328085..328267	+	183	WP_000923340.1	DUF645 family protein	-
ASZ80_RS15735	328482..329021	+	540	WP_000099372.1	hypothetical protein	-
ASZ80_RS15740	329144..329659	+	516	WP_000090011.1	hypothetical protein	-
ASZ80_RS15745	329794..330330	+	537	WP_000644491.1	nucleotidyltransferase family protein	-
ASZ80_RS20940	330449..330610	+	162	WP_000481782.1	hypothetical protein	-
ASZ80_RS15750	330799..330909	+	111	WP_071908335.1	DUF3265 domain-containing protein	-
ASZ80_RS15760	331122..331274	+	153	WP_001884520.1	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304694..469286	164592
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.005	inside	Integron	-	-	309774..468910	159136

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-89)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18539.23 Da
• Isoelectric Point: 8.7753

>T58213 WP_000982256.1 NZ_CP013302:c326021-325524 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGIEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Nucleotide

• Length: 498 bp

>T58213 NZ_CP013302:c326021-325524 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCATTGAAGCCCTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACACCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCTTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCGCTCAGAAAGCTACTCGCAGCCAGTGACAGCGTAGCGTTCCCTGTTGTGATCGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCTGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9685.19 Da
• Isoelectric Point: 6.2988

>AT58213 WP_000246254.1 NZ_CP013302:c326290-326018 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLTKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVANAKLKL
ASERYESKTQ

Nucleotide

• Length: 273 bp

>AT58213 NZ_CP013302:c326290-326018 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCACTAAGGCTGCTGCTCTTGC
TGGCATGTCTAGTATTAACTCATTTGTTTTGAATGCGGCAATTGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCGGTAGCAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Structures

Toxin

• AlphaFold DB: A0A233HKB4