Detailed information of TA system TA056912

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 332541..333039
• Replicon: chromosome
• Accession: NZ_CP012666
• Organism: Vibrio cholerae strain ICDC-VC661

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: AN947_RS01650
• Protein ID: WP_000589156.1
• Coordinates: 332541..332807 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: AN947_RS01655
• Protein ID: WP_000643598.1
• Coordinates: 332794..333039 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN947_RS20720	327962..328168	+	207	Protein_290	DUF3709 domain-containing protein	-
AN947_RS20725	328368..328469	+	102	WP_001921603.1	hypothetical protein	-
AN947_RS01590	328459..328845	+	387	WP_000703163.1	VOC family protein	-
AN947_RS01595	329040..329291	+	252	WP_001890111.1	TIGR03643 family protein	-
AN947_RS01600	329264..329413	+	150	WP_071908333.1	DUF3265 domain-containing protein	-
AN947_RS01605	329505..329747	+	243	WP_000107461.1	hypothetical protein	-
AN947_RS01610	329999..330277	+	279	WP_000280324.1	DUF3709 domain-containing protein	-
AN947_RS20730	330652..330795	+	144	Protein_297	DUF645 family protein	-
AN947_RS20735	330849..330887	+	39	WP_082798268.1	hypothetical protein	-
AN947_RS01635	331099..331566	+	468	WP_001289288.1	OsmC family protein	-
AN947_RS01640	331694..332356	+	663	WP_000960654.1	hypothetical protein	-
AN947_RS01650	332541..332807	+	267	WP_000589156.1	BrnT family toxin	Toxin
AN947_RS01655	332794..333039	+	246	WP_000643598.1	hypothetical protein	Antitoxin
AN947_RS01665	333135..333929	+	795	WP_001911581.1	hypothetical protein	-
AN947_RS01675	334032..334160	+	129	WP_071908338.1	general secretion pathway protein GspI	-
AN947_RS01680	334221..334403	+	183	WP_000923182.1	DUF645 family protein	-
AN947_RS01685	334603..335076	+	474	WP_001161076.1	GrpB family protein	-
AN947_RS01690	335091..335183	+	93	WP_014378730.1	DUF3265 domain-containing protein	-
AN947_RS01695	335225..335851	+	627	WP_000365424.1	LysE family translocator	-
AN947_RS01700	335974..336672	+	699	WP_001890502.1	hypothetical protein	-
AN947_RS01705	336682..336792	+	111	WP_079857360.1	DUF3265 domain-containing protein	-
AN947_RS01720	337370..337549	+	180	WP_001883058.1	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304645..399892	95247
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.005	inside	Integron	-	-	309725..399516	89791

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T56912 WP_000589156.1 NZ_CP012666:332541-332807 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T56912 NZ_CP012666:332541-332807 [Vibrio cholerae]
ATGATTAAATTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAGCACGGTATTGATTTTCATACAGCTCAAGG
ATTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAACACGAGTGATGAGCCCAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGAGTAATTACCTACCGAGGTACGAATATCAGGATCATCTCAGTTCGGCGTTCACGGAAAGCG
GAGGTAAGCCTTTATGAAAGCGCATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT56912 WP_000643598.1 NZ_CP012666:332794-333039 [Vibrio cholerae]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT56912 NZ_CP012666:332794-333039 [Vibrio cholerae]
ATGAAAGCGCATGAATTTGATGCCAAGTTTGAAAGTGACGACGATGATATCGTAATGGACTTGGATCTATCGCAAGCTAA
AAGACCGATGCATAAGCAAAAACGAGTCAATGTAGATTTTCCAGCCTGGATGCTTGAGTCCTTGGACAGAGAAGCGAGTC
GTATTGGTGTAACACGTCAATCAATCATTAAAATTTGGCTGGCAGAAAGGTTAGAGAGCGTGTCTCATCATTCAAGTGTG
AGATAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: A0A271VME2