Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 422310..422838 Replicon chromosome
Accession NZ_CP028828
Organism Vibrio cholerae strain N16961

Toxin (Protein)


Gene name relE Uniprot ID A0A0X1KZS6
Locus tag N16961_RS16570 Protein ID WP_000221354.1
Coordinates 422551..422838 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0X1KZS8
Locus tag N16961_RS16565 Protein ID WP_001250179.1
Coordinates 422310..422561 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N16961_RS16525 417578..417892 + 315 WP_000071008.1 DNA-binding transcriptional regulator -
N16961_RS16530 418029..418463 + 435 WP_000256036.1 GNAT family N-acetyltransferase -
N16961_RS19670 418631..418786 + 156 WP_000751734.1 hypothetical protein -
N16961_RS16535 418911..419891 - 981 WP_000019370.1 IS5-like element ISVch5 family transposase -
N16961_RS16540 419959..420265 + 307 Protein_448 CatB-related O-acetyltransferase -
N16961_RS16545 420402..421094 + 693 WP_001047169.1 GNAT family N-acetyltransferase -
N16961_RS16550 421094..421495 + 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin -
N16961_RS16555 421465..421608 + 144 WP_071908341.1 DUF3265 domain-containing protein -
N16961_RS16560 421683..422096 + 414 WP_000049417.1 VOC family protein -
N16961_RS16565 422310..422561 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
N16961_RS16570 422551..422838 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
N16961_RS16575 422966..423421 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
N16961_RS16580 423743..423895 + 153 WP_001884520.1 DUF645 family protein -
N16961_RS16590 424097..424594 - 498 WP_000982260.1 GNAT family N-acetyltransferase -
N16961_RS16595 424591..424863 - 273 WP_000246253.1 DUF1778 domain-containing protein -
N16961_RS16600 425094..425762 + 669 WP_000043871.1 hypothetical protein -
N16961_RS16605 425914..426201 + 288 WP_000426470.1 hypothetical protein -
N16961_RS16610 426342..426713 + 372 WP_001164080.1 nucleotide pyrophosphohydrolase -
N16961_RS16615 426780..427205 + 426 WP_001882332.1 DUF1778 domain-containing protein -
N16961_RS16620 427202..427735 + 534 WP_000118351.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 306977..438073 131096
inside Integron - - 312057..437697 125640
flank IS/Tn - - 418911..419891 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(11-71)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 10991.96 Da        Isoelectric Point: 10.4934

>T104258 WP_000221354.1 NZ_CP028828:422551-422838 [Vibrio cholerae]
MTYKLKFLPAAQKEWSKLAPTIQSQFKKKLKERLENPHVPSAKLRGYDAVYKIKLRTAGYRLAYEVIDDEIVVYVLAVGK
RDKDAVYKKLASRFG

Download         Length: 288 bp

>T104258 NZ_CP028828:422551-422838 [Vibrio cholerae]
ATGACCTATAAGTTAAAGTTTCTGCCGGCTGCGCAAAAGGAATGGAGTAAGTTAGCTCCGACAATTCAGAGTCAGTTCAA
GAAGAAGTTAAAGGAACGATTAGAAAATCCGCACGTCCCTTCGGCTAAGCTTCGAGGATACGACGCTGTTTACAAGATTA
AACTTCGTACGGCGGGTTATCGTTTAGCTTATGAGGTTATTGACGATGAGATAGTTGTCTATGTCCTTGCTGTCGGTAAA
CGTGACAAAGATGCTGTCTATAAAAAACTGGCTTCACGCTTCGGTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9136.29 Da        Isoelectric Point: 4.0197

>AT104258 WP_001250179.1 NZ_CP028828:422310-422561 [Vibrio cholerae]
MRQVLANCSASISELKKNPTALLNEADGSAIAILNHNKPAAYLVPAETYEYLIDMLDDYELSQIVDSRRADLAQAVEVNI
DDL

Download         Length: 252 bp

>AT104258 NZ_CP028828:422310-422561 [Vibrio cholerae]
ATGAGACAAGTTTTAGCGAATTGCTCTGCAAGTATTTCAGAGTTAAAGAAGAACCCAACAGCTTTGTTGAATGAGGCTGA
TGGGTCTGCAATTGCAATTTTAAACCACAACAAGCCAGCGGCTTACCTTGTGCCAGCGGAAACGTATGAGTATCTCATCG
ATATGCTCGATGATTATGAGCTTTCCCAAATTGTCGATAGTCGCCGAGCTGACTTAGCACAAGCTGTGGAAGTAAATATT
GATGACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0X1KZS6

Antitoxin

Source ID Structure
AlphaFold DB A0A0X1KZS8

References