58427

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 422262..423028 Replicon chromosome
Accession NZ_CP013320
Organism Vibrio cholerae strain NCTC9420

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag ASZ86_RS16715 Protein ID WP_000982260.1
Coordinates 422262..422759 (-) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9K2J6
Locus tag ASZ86_RS16720 Protein ID WP_000246253.1
Coordinates 422756..423028 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ASZ86_RS16665 418124..418430 + 307 Protein_447 CatB-related O-acetyltransferase -
ASZ86_RS16670 418567..419259 + 693 WP_001047169.1 GNAT family N-acetyltransferase -
ASZ86_RS16675 419259..419660 + 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin -
ASZ86_RS20545 419630..419773 + 144 WP_071908341.1 DUF3265 domain-containing protein -
ASZ86_RS16685 419848..420261 + 414 WP_000049417.1 VOC family protein -
ASZ86_RS16690 420475..420726 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
ASZ86_RS16695 420716..421003 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin -
ASZ86_RS16700 421131..421586 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
ASZ86_RS16710 421908..422060 + 153 WP_001884520.1 DUF645 family protein -
ASZ86_RS16715 422262..422759 - 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
ASZ86_RS16720 422756..423028 - 273 WP_000246253.1 DUF1778 domain-containing protein Antitoxin
ASZ86_RS16725 423259..423927 + 669 WP_000043871.1 hypothetical protein -
ASZ86_RS16730 424079..424366 + 288 WP_000426470.1 hypothetical protein -
ASZ86_RS16735 424507..424878 + 372 WP_001164080.1 nucleotide pyrophosphohydrolase -
ASZ86_RS16740 424945..425370 + 426 WP_001882332.1 DUF1778 domain-containing protein -
ASZ86_RS16745 425367..425900 + 534 WP_000118351.1 GNAT family N-acetyltransferase -
ASZ86_RS16750 426102..426359 + 258 WP_000861987.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
ASZ86_RS16755 426347..426649 + 303 WP_000229317.1 type II toxin-antitoxin system RelE/ParE family toxin -
ASZ86_RS16760 426883..427800 + 918 WP_000186333.1 alpha/beta hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 304702..436238 131536
- inside Integron - - 309782..435862 126080
flank IS/Tn - - 417076..418056 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T58427 WP_000982260.1 NZ_CP013320:c422759-422262 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T58427 NZ_CP013320:c422759-422262 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9683.22 Da        Isoelectric Point: 6.2988

>AT58427 WP_000246253.1 NZ_CP013320:c423028-422756 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT58427 NZ_CP013320:c423028-422756 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
AlphaFold DB A0A0F2IC79

References