Detailed information of TA system TA058360

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 402126..402741
• Replicon: chromosome
• Accession: NZ_CP013314
• Organism: Vibrio cholerae strain E9120

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: ASZ79_RS16400
• Protein ID: WP_001162670.1
• Coordinates: 402126..402413 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ASZ79_RS16405
• Protein ID: WP_001232701.1
• Coordinates: 402424..402741 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ79_RS21005	397762..397857	+	96	WP_001907607.1	DUF645 family protein	-
ASZ79_RS16360	398051..398368	-	318	WP_001180243.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ79_RS16365	398387..398629	-	243	WP_000557292.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ79_RS16370	398871..399380	+	510	WP_000405987.1	GNAT family N-acetyltransferase	-
ASZ79_RS16375	399437..400090	+	654	WP_000226874.1	hypothetical protein	-
ASZ79_RS16380	400400..400618	+	219	WP_001917086.1	DUF3709 domain-containing protein	-
ASZ79_RS16390	401021..401254	+	234	WP_032482776.1	DUF3709 domain-containing protein	-
ASZ79_RS16395	401679..401859	+	181	Protein_416	DUF645 family protein	-
ASZ79_RS16400	402126..402413	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ASZ79_RS16405	402424..402741	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
ASZ79_RS21010	402738..402848	+	111	WP_134814058.1	DUF3265 domain-containing protein	-
ASZ79_RS21015	403025..403150	+	126	WP_001944767.1	DUF645 family protein	-
ASZ79_RS21020	403166..403204	+	39	WP_106019119.1	hypothetical protein	-
ASZ79_RS16425	403428..404168	+	741	WP_000469836.1	PhzF family phenazine biosynthesis protein	-
ASZ79_RS16430	404373..405203	+	831	WP_000056616.1	abortive infection family protein	-
ASZ79_RS16435	405260..405742	+	483	WP_001895003.1	hypothetical protein	-
ASZ79_RS16445	406189..407064	+	876	WP_000259044.1	hypothetical protein	-
ASZ79_RS16450	407194..407649	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304700..468464	163764
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.005	inside	Integron	-	-	309780..468088	158308

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T58360 WP_001162670.1 NZ_CP013314:402126-402413 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T58360 NZ_CP013314:402126-402413 [Vibrio cholerae]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT58360 WP_001232701.1 NZ_CP013314:402424-402741 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT58360 NZ_CP013314:402424-402741 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36