Detailed information of TA system TA058404

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-GNAT
• Location: 479162..480255
• Replicon: chromosome
• Accession: NZ_CP013318
• Organism: Vibrio cholerae strain NCTC5395

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9KMA2
• Locus tag: ASZ85_RS17055
• Protein ID: WP_000351248.1
• Coordinates: 479854..480255 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: ASZ85_RS17050
• Protein ID: WP_001047169.1
• Coordinates: 479162..479854 (+)
• Length: 231 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ85_RS16980	474445..474822	+	378	WP_000411109.1	hypothetical protein	-
ASZ85_RS21485	474879..474993	+	115	Protein_520	acetyltransferase	-
ASZ85_RS16985	474942..475223	+	282	WP_001894453.1	DUF1289 domain-containing protein	-
ASZ85_RS17000	475389..475502	+	114	WP_001889158.1	hypothetical protein	-
ASZ85_RS17005	475451..475678	+	228	WP_001894457.1	DUF1289 domain-containing protein	-
ASZ85_RS17015	476019..476351	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ85_RS17020	476338..476652	+	315	WP_000071008.1	DNA-binding transcriptional regulator	-
ASZ85_RS17025	476789..477223	+	435	WP_000256036.1	GNAT family N-acetyltransferase	-
ASZ85_RS21305	477391..477546	+	156	WP_000751734.1	hypothetical protein	-
ASZ85_RS17040	477671..478651	-	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
ASZ85_RS17045	478719..479025	+	307	Protein_529	CatB-related O-acetyltransferase	-
ASZ85_RS17050	479162..479854	+	693	WP_001047169.1	GNAT family N-acetyltransferase	Antitoxin
ASZ85_RS17055	479854..480255	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
ASZ85_RS21215	480225..480368	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
ASZ85_RS17065	480443..480856	+	414	WP_000049417.1	VOC family protein	-
ASZ85_RS17070	481070..481321	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ85_RS17075	481311..481598	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ85_RS17080	481726..482181	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
ASZ85_RS17090	482503..482655	+	153	WP_001884520.1	DUF645 family protein	-
ASZ85_RS17095	482857..483354	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
ASZ85_RS17100	483351..483623	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
ASZ85_RS17105	483854..484522	+	669	WP_000043871.1	hypothetical protein	-
ASZ85_RS17110	484674..484961	+	288	WP_000426470.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	338285..496833	158548
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.009	inside	Integron	-	-	343365..496457	153092
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	477671..478651	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-132)

Antitoxin

• GNAT domain (36-153)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14608.71 Da
• Isoelectric Point: 4.0128

>T58404 WP_000351248.1 NZ_CP013318:479854-480255 [Vibrio cholerae]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Nucleotide

• Length: 402 bp

>T58404 NZ_CP013318:479854-480255 [Vibrio cholerae]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin

Protein

• Length: 231 a.a.
• Molecular weight: 26352.73 Da
• Isoelectric Point: 8.5633

>AT58404 WP_001047169.1 NZ_CP013318:479162-479854 [Vibrio cholerae]
MNLEEFQESDFDLLIKWIDSDELNYLWGCPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 693 bp

>AT58404 NZ_CP013318:479162-479854 [Vibrio cholerae]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTTGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTATGGTTGTTGTGATTGAGTTTGGCGGTATGCGTTGTCAGCCCCTTAGGCGGGCGTTATGTTTTTTATCACGTTTT
GGGGCTATAATCGGCCTAACCTTTATTGATAGTAGGTGTGATATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGT
TGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGACTGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTA
AACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGGCTGATATGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q0PN34