Detailed information of TA system TA145867

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 333842..334340
• Replicon: chromosome
• Accession: NZ_CP047302
• Organism: Vibrio cholerae O1 biovar El Tor strain DRC-193A

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: GTF75_RS15425
• Protein ID: WP_000589156.1
• Coordinates: 333842..334108 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: GTF75_RS15430
• Protein ID: WP_000643598.1
• Coordinates: 334095..334340 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTF75_RS15370	329263..329469	+	207	Protein_293	DUF3709 domain-containing protein	-
GTF75_RS15375	329669..329770	+	102	WP_001921603.1	hypothetical protein	-
GTF75_RS15380	329760..330146	+	387	WP_000703163.1	VOC family protein	-
GTF75_RS15385	330341..330592	+	252	WP_001890111.1	TIGR03643 family protein	-
GTF75_RS15390	330565..330714	+	150	WP_071908333.1	DUF3265 domain-containing protein	-
GTF75_RS15395	330806..331048	+	243	WP_000107461.1	hypothetical protein	-
GTF75_RS15400	331300..331578	+	279	WP_000280324.1	DUF3709 domain-containing protein	-
GTF75_RS15405	331953..332096	+	144	Protein_300	DUF645 family protein	-
GTF75_RS15410	332150..332188	+	39	WP_082798268.1	hypothetical protein	-
GTF75_RS15415	332400..332867	+	468	WP_001289288.1	OsmC family protein	-
GTF75_RS15420	332995..333657	+	663	WP_000960654.1	hypothetical protein	-
GTF75_RS15425	333842..334108	+	267	WP_000589156.1	BrnT family toxin	Toxin
GTF75_RS15430	334095..334340	+	246	WP_000643598.1	hypothetical protein	Antitoxin
GTF75_RS15435	334436..335230	+	795	WP_001911581.1	hypothetical protein	-
GTF75_RS15440	335333..335461	+	129	WP_071908338.1	general secretion pathway protein GspI	-
GTF75_RS15445	335522..335704	+	183	WP_000923182.1	DUF645 family protein	-
GTF75_RS15450	335920..336924	+	1005	WP_000964920.1	LD-carboxypeptidase	-
GTF75_RS15455	337077..337436	+	360	WP_001071541.1	VOC family protein	-
GTF75_RS15460	337709..337942	+	234	WP_001890112.1	DUF3709 domain-containing protein	-
GTF75_RS15465	338210..338716	+	507	WP_000393074.1	hypothetical protein	-
GTF75_RS15470	338873..339259	+	387	WP_000703163.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	305935..411136	105201
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	-	-	311015..410760	99745

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T145867 WP_000589156.1 NZ_CP047302:333842-334108 [Vibrio cholerae O1 biovar El Tor]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T145867 NZ_CP062705:c710184-709720 [Escherichia coli O157:H7]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT145867 WP_000643598.1 NZ_CP047302:334095-334340 [Vibrio cholerae O1 biovar El Tor]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT145867 NZ_CP062705:c710519-710184 [Escherichia coli O157:H7]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: A0A271VME2