145853

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-MqsA
Location 418478..419111 Replicon chromosome
Accession NZ_CP047300
Organism Vibrio cholerae O1 biovar El Tor strain P27459

Toxin (Protein)


Gene name higB Uniprot ID Q9KMA6
Locus tag GTF71_RS15700 Protein ID WP_000843587.1
Coordinates 418478..418810 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag GTF71_RS15705 Protein ID WP_000071008.1
Coordinates 418797..419111 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTF71_RS15635 413919..414122 + 204 WP_001911745.1 hypothetical protein -
GTF71_RS15640 414403..414576 + 174 WP_001882305.1 DUF3709 domain-containing protein -
GTF71_RS15645 414936..415205 + 270 WP_001198131.1 hypothetical protein -
GTF71_RS15650 415516..415602 + 87 WP_134820423.1 DUF645 family protein -
GTF71_RS15655 415602..415694 + 93 Protein_441 DUF645 family protein -
GTF71_RS15660 415903..416289 + 387 WP_000703163.1 VOC family protein -
GTF71_RS15665 416491..416733 + 243 WP_000107461.1 hypothetical protein -
GTF71_RS15670 416904..417281 + 378 WP_000411109.1 hypothetical protein -
GTF71_RS15675 417461..417682 + 222 WP_032468461.1 DUF1289 domain-containing protein -
GTF71_RS15680 417658..417816 - 159 WP_001894455.1 DUF1196 family protein -
GTF71_RS15685 417848..417961 + 114 WP_001889158.1 hypothetical protein -
GTF71_RS15690 417910..418137 + 228 WP_001894457.1 DUF1289 domain-containing protein -
GTF71_RS15695 418167..418325 - 159 WP_001882309.1 DUF1196 family protein -
GTF71_RS15700 418478..418810 + 333 WP_000843587.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GTF71_RS15705 418797..419111 + 315 WP_000071008.1 DNA-binding transcriptional regulator Antitoxin
GTF71_RS15710 419248..419682 + 435 WP_000256036.1 GNAT family N-acetyltransferase -
GTF71_RS15715 419850..420005 + 156 WP_000751734.1 hypothetical protein -
GTF71_RS15720 420130..421110 - 981 WP_000019370.1 IS5-like element ISVch5 family transposase -
GTF71_RS15725 421178..421450 + 273 Protein_455 CatB-related O-acetyltransferase -
GTF71_RS15730 421621..422313 + 693 WP_001047169.1 GNAT family N-acetyltransferase -
GTF71_RS15735 422313..422714 + 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin -
GTF71_RS15740 422684..422827 + 144 WP_071908341.1 DUF3265 domain-containing protein -
GTF71_RS15745 422902..423315 + 414 WP_000049417.1 VOC family protein -
GTF71_RS15750 423529..423780 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
GTF71_RS15755 423770..424057 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 306926..439292 132366
inside Integron - - 312006..438916 126910
flank IS/Tn - - 420130..421110 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(4-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(43-92)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 13005.95 Da        Isoelectric Point: 9.9244

>T145853 WP_000843587.1 NZ_CP047300:418478-418810 [Vibrio cholerae O1 biovar El Tor]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS

Download         Length: 333 bp

>T145853 NZ_CP062702:4415541-4415801 [Escherichia coli O157:H7]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11696.20 Da        Isoelectric Point: 6.7600

>AT145853 WP_000071008.1 NZ_CP047300:418797-419111 [Vibrio cholerae O1 biovar El Tor]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL

Download         Length: 315 bp

>AT145853 NZ_CP062702:4415262-4415522 [Escherichia coli O157:H7]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5JA9
PDB 8A0X
PDB 5MJE
PDB 5JA8
PDB 5JAA

Antitoxin

Source ID Structure

References