48152

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 368082..368697 Replicon chromosome
Accession NZ_CP009041
Organism Vibrio cholerae O1 biovar El Tor strain FJ147

Toxin (Protein)


Gene name higB Uniprot ID Q9KMG5
Locus tag IR04_RS01795 Protein ID WP_001162670.1
Coordinates 368082..368369 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag IR04_RS01800 Protein ID WP_001232701.1
Coordinates 368380..368697 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IR04_RS20395 363718..363813 + 96 WP_001907607.1 DUF645 family protein -
IR04_RS01765 364007..364324 - 318 WP_001180243.1 type II toxin-antitoxin system RelE/ParE family toxin -
IR04_RS01770 364343..364585 - 243 WP_000557292.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
IR04_RS01775 364827..365336 + 510 WP_000405987.1 GNAT family N-acetyltransferase -
IR04_RS01780 365393..366046 + 654 WP_000226874.1 hypothetical protein -
IR04_RS01785 366356..366574 + 219 WP_001917086.1 DUF3709 domain-containing protein -
IR04_RS19310 366977..367210 + 234 WP_032482776.1 DUF3709 domain-containing protein -
IR04_RS01790 367635..367815 + 181 Protein_361 DUF645 family protein -
IR04_RS01795 368082..368369 + 288 WP_001162670.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IR04_RS01800 368380..368697 + 318 WP_001232701.1 HigA family addiction module antidote protein Antitoxin
IR04_RS20400 368694..368804 + 111 WP_134814058.1 DUF3265 domain-containing protein -
IR04_RS20405 368981..369106 + 126 WP_001944767.1 DUF645 family protein -
IR04_RS20410 369122..369160 + 39 WP_106019119.1 hypothetical protein -
IR04_RS01805 369373..370377 + 1005 WP_000964925.1 LD-carboxypeptidase -
IR04_RS01810 370530..370976 + 447 WP_000128977.1 hypothetical protein -
IR04_RS01815 371096..372025 + 930 WP_000335802.1 hypothetical protein -
IR04_RS19990 372022..372132 + 111 WP_071917832.1 DUF3265 domain-containing protein -
IR04_RS01820 372160..372636 + 477 WP_001047180.1 GNAT family N-acetyltransferase -
IR04_RS01825 372773..373288 + 516 WP_001201528.1 lipocalin family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 304700..409892 105192
inside Integron - - 309780..409516 99736


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(2-95)

Antitoxin

Cro/C1-type helix-turn-helix domain

(12-69)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11349.95 Da        Isoelectric Point: 6.7187

>T48152 WP_001162670.1 NZ_CP009041:368082-368369 [Vibrio cholerae O1 biovar El Tor]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Download         Length: 288 bp

>T48152 NZ_CP009041:368082-368369 [Vibrio cholerae O1 biovar El Tor]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11705.63 Da        Isoelectric Point: 10.4711

>AT48152 WP_001232701.1 NZ_CP009041:368380-368697 [Vibrio cholerae O1 biovar El Tor]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Download         Length: 318 bp

>AT48152 NZ_CP009041:368380-368697 [Vibrio cholerae O1 biovar El Tor]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F2HI36

Antitoxin

Source ID Structure

References