Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/RelE-MqsA |
| Location | 424200..424833 | Replicon | chromosome II |
| Accession | NC_012583 | ||
| Organism | Vibrio cholerae O395 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | Q9KMA6 |
| Locus tag | VC395_RS16415 | Protein ID | WP_000843587.1 |
| Coordinates | 424200..424532 (+) | Length | 111 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | VC395_RS16420 | Protein ID | WP_000071008.1 |
| Coordinates | 424519..424833 (+) | Length | 105 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| VC395_RS16385 | 419826..420683 | + | 858 | WP_001279526.1 | DUF2806 domain-containing protein | - |
| VC395_RS16390 | 420839..421390 | + | 552 | WP_000821930.1 | hypothetical protein | - |
| VC395_RS21055 | 421677..421880 | + | 204 | WP_001911745.1 | hypothetical protein | - |
| VC395_RS20925 | 422161..422334 | + | 174 | WP_001882305.1 | DUF3709 domain-containing protein | - |
| VC395_RS16400 | 422694..422963 | + | 270 | WP_001198131.1 | hypothetical protein | - |
| VC395_RS21115 | 423281..423452 | + | 172 | Protein_431 | DUF645 family protein | - |
| VC395_RS20375 | 423569..423683 | + | 115 | Protein_432 | acetyltransferase | - |
| VC395_RS16405 | 423632..423859 | + | 228 | WP_001894457.1 | DUF1289 domain-containing protein | - |
| VC395_RS16415 | 424200..424532 | + | 333 | WP_000843587.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| VC395_RS16420 | 424519..424833 | + | 315 | WP_000071008.1 | DNA-binding transcriptional regulator | Antitoxin |
| VC395_RS16425 | 424970..425404 | + | 435 | WP_000256036.1 | GNAT family N-acetyltransferase | - |
| VC395_RS21070 | 425572..425727 | + | 156 | WP_000751734.1 | hypothetical protein | - |
| VC395_RS16435 | 425852..426832 | - | 981 | WP_000019370.1 | IS5-like element ISVch5 family transposase | - |
| VC395_RS16440 | 426900..427172 | + | 273 | Protein_439 | CatB-related O-acetyltransferase | - |
| VC395_RS16445 | 427343..428035 | + | 693 | WP_001047169.1 | GNAT family N-acetyltransferase | - |
| VC395_RS16450 | 428035..428436 | + | 402 | WP_000351248.1 | type II toxin-antitoxin system death-on-curing family toxin | - |
| VC395_RS20655 | 428406..428549 | + | 144 | WP_071908341.1 | DUF3265 domain-containing protein | - |
| VC395_RS16455 | 428624..429037 | + | 414 | WP_000049417.1 | VOC family protein | - |
| VC395_RS16460 | 429251..429502 | + | 252 | WP_001250179.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | - |
| VC395_RS16465 | 429492..429779 | + | 288 | WP_000221354.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integron | - | - | 344619..430484 | 85865 | |
| flank | IS/Tn | - | - | 425852..426832 | 980 | ||
| - | inside | Prophage | - | - | 427343..471178 | 43835 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 111 a.a. Molecular weight: 13005.95 Da Isoelectric Point: 9.9244
>T23732 WP_000843587.1 NC_012583:424200-424532 [Vibrio cholerae O395]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS
Download Length: 333 bp
>T23732 NC_012583:424200-424532 [Vibrio cholerae O395]
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGGCGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGGCGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA
Antitoxin
Download Length: 105 a.a. Molecular weight: 11696.20 Da Isoelectric Point: 6.7600
>AT23732 WP_000071008.1 NC_012583:424519-424833 [Vibrio cholerae O395]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL
Download Length: 315 bp
>AT23732 NC_012583:424519-424833 [Vibrio cholerae O395]
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCGCGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCGCGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|