38675

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-MqsA
Location 232924..233557 Replicon chromosome
Accession NZ_AP024554
Organism Vibrio cholerae strain IDH-03329

Toxin (Protein)


Gene name higB Uniprot ID Q9KMA6
Locus tag K6J80_RS14970 Protein ID WP_000843587.1
Coordinates 233225..233557 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag K6J80_RS14965 Protein ID WP_000071008.1
Coordinates 232924..233238 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J80_RS14920 227978..228265 - 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin -
K6J80_RS14925 228255..228506 - 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
K6J80_RS14930 228720..229133 - 414 WP_000049417.1 VOC family protein -
K6J80_RS18960 229208..229318 - 111 WP_082798255.1 DUF3265 domain-containing protein -
K6J80_RS14935 229321..229722 - 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin -
K6J80_RS18965 229722..229892 - 171 WP_001080654.1 hypothetical protein -
K6J80_RS18970 230016..230414 - 399 Protein_219 GNAT family N-acetyltransferase -
K6J80_RS14945 230551..230857 - 307 Protein_220 CatB-related O-acetyltransferase -
K6J80_RS14950 230925..231905 + 981 WP_000019370.1 IS5-like element ISVch5 family transposase -
K6J80_RS14955 232030..232185 - 156 WP_000751734.1 hypothetical protein -
K6J80_RS14960 232353..232787 - 435 WP_000256036.1 GNAT family N-acetyltransferase -
K6J80_RS14965 232924..233238 - 315 WP_000071008.1 DNA-binding transcriptional regulator Antitoxin
K6J80_RS14970 233225..233557 - 333 WP_000843587.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K6J80_RS14975 233898..234125 - 228 WP_073426587.1 DUF1289 domain-containing protein -
K6J80_RS14980 234074..234187 - 114 WP_001889158.1 hypothetical protein -
K6J80_RS14985 234353..234574 - 222 WP_032468461.1 DUF1289 domain-containing protein -
K6J80_RS14990 234583..234697 - 115 Protein_229 acetyltransferase -
K6J80_RS14995 234754..235131 - 378 WP_000411109.1 hypothetical protein -
K6J80_RS15000 235302..235661 - 360 WP_226979446.1 pullulanase -
K6J80_RS15005 235746..236132 - 387 WP_000703163.1 VOC family protein -
K6J80_RS15010 236341..236473 - 133 Protein_233 DUF645 family protein -
K6J80_RS18975 236464..236589 - 126 WP_080360924.1 general secretion pathway protein GspH -
K6J80_RS15015 236830..237099 - 270 WP_001198131.1 hypothetical protein -
K6J80_RS15020 237459..237656 - 198 WP_001894451.1 DUF3709 domain-containing protein -
K6J80_RS15025 237913..238116 - 204 WP_001911745.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 213119..319686 106567
inside Integron - - 213119..312856 99737
flank IS/Tn - - 230925..231905 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(4-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(43-92)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 13005.95 Da        Isoelectric Point: 9.9244

>T38675 WP_000843587.1 NZ_AP024554:c233557-233225 [Vibrio cholerae]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS

Download         Length: 333 bp

>T38675 NZ_AP024554:c233557-233225 [Vibrio cholerae]
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGGCGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11696.20 Da        Isoelectric Point: 6.7600

>AT38675 WP_000071008.1 NZ_AP024554:c233238-232924 [Vibrio cholerae]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL

Download         Length: 315 bp

>AT38675 NZ_AP024554:c233238-232924 [Vibrio cholerae]
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCGCGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5JA9
PDB 8A0X
PDB 5MJE
PDB 5JA8
PDB 5JAA

Antitoxin

Source ID Structure

References