Detailed information of TA system TA104250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 336260..336758
• Replicon: chromosome
• Accession: NZ_CP028828
• Organism: Vibrio cholerae strain N16961

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: N16961_RS15650
• Protein ID: WP_000589156.1
• Coordinates: 336260..336526 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: N16961_RS15655
• Protein ID: WP_000643598.1
• Coordinates: 336513..336758 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N16961_RS15590	331681..331887	+	207	Protein_294	DUF3709 domain-containing protein	-
N16961_RS15595	332087..332188	+	102	WP_001921603.1	hypothetical protein	-
N16961_RS15600	332178..332564	+	387	WP_000703163.1	VOC family protein	-
N16961_RS15605	332759..333010	+	252	WP_001890111.1	TIGR03643 family protein	-
N16961_RS15610	332983..333132	+	150	WP_071908333.1	DUF3265 domain-containing protein	-
N16961_RS15615	333224..333466	+	243	WP_000107461.1	hypothetical protein	-
N16961_RS15620	333718..333996	+	279	WP_000280324.1	DUF3709 domain-containing protein	-
N16961_RS19805	334371..334514	+	144	Protein_301	DUF645 family protein	-
N16961_RS19810	334568..334606	+	39	WP_082798268.1	hypothetical protein	-
N16961_RS15635	334818..335285	+	468	WP_001289288.1	OsmC family protein	-
N16961_RS15640	335413..336075	+	663	WP_000960654.1	hypothetical protein	-
N16961_RS15650	336260..336526	+	267	WP_000589156.1	BrnT family toxin	Toxin
N16961_RS15655	336513..336758	+	246	WP_000643598.1	hypothetical protein	Antitoxin
N16961_RS15660	336854..337648	+	795	WP_001911581.1	hypothetical protein	-
N16961_RS19825	337751..337879	+	129	WP_071908338.1	general secretion pathway protein GspI	-
N16961_RS15675	337940..338122	+	183	WP_000923182.1	DUF645 family protein	-
N16961_RS15680	338338..339342	+	1005	WP_000964920.1	LD-carboxypeptidase	-
N16961_RS15685	339495..339854	+	360	WP_001071541.1	VOC family protein	-
N16961_RS15690	340127..340360	+	234	WP_001890112.1	DUF3709 domain-containing protein	-
N16961_RS15695	340628..341134	+	507	WP_000393074.1	hypothetical protein	-
N16961_RS15700	341291..341677	+	387	WP_000703163.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	306977..438073	131096
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0	inside	Integron	-	-	312057..437697	125640

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T104250 WP_000589156.1 NZ_CP028828:336260-336526 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T104250 NZ_CP028828:336260-336526 [Vibrio cholerae]
ATGATTAAATTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAGCACGGTATTGATTTTCATACAGCTCAAGG
ATTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAACACGAGTGATGAGCCCAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGAGTAATTACCTACCGAGGTACGAATATCAGGATCATCTCAGTTCGGCGTTCACGGAAAGCG
GAGGTAAGCCTTTATGAAAGCGCATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT104250 WP_000643598.1 NZ_CP028828:336513-336758 [Vibrio cholerae]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT104250 NZ_CP028828:336513-336758 [Vibrio cholerae]
ATGAAAGCGCATGAATTTGATGCCAAGTTTGAAAGTGACGACGATGATATCGTAATGGACTTGGATCTATCGCAAGCTAA
AAGACCGATGCATAAGCAAAAACGAGTCAATGTAGATTTTCCAGCCTGGATGCTTGAGTCCTTGGACAGAGAAGCGAGTC
GTATTGGTGTAACACGTCAATCAATCATTAAAATTTGGCTGGCAGAAAGGTTAGAGAGCGTGTCTCATCATTCAAGTGTG
AGATAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: A0A271VME2