Detailed information of TA system TA145877

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 399843..400798
• Replicon: chromosome
• Accession: NZ_CP047302
• Organism: Vibrio cholerae O1 biovar El Tor strain DRC-193A

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KM94
• Locus tag: GTF75_RS16030
• Protein ID: WP_000118351.1
• Coordinates: 400265..400798 (+)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: GTF75_RS16025
• Protein ID: WP_001882332.1
• Coordinates: 399843..400268 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTF75_RS15980	395373..395624	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
GTF75_RS15985	395614..395901	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GTF75_RS15990	396029..396484	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
GTF75_RS15995	396806..396958	+	153	WP_001884520.1	DUF645 family protein	-
GTF75_RS16000	397160..397657	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
GTF75_RS16005	397654..397926	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
GTF75_RS16010	398157..398825	+	669	WP_000043871.1	hypothetical protein	-
GTF75_RS16015	398977..399264	+	288	WP_000426470.1	hypothetical protein	-
GTF75_RS16020	399405..399776	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
GTF75_RS16025	399843..400268	+	426	WP_001882332.1	DUF1778 domain-containing protein	Antitoxin
GTF75_RS16030	400265..400798	+	534	WP_000118351.1	GNAT family N-acetyltransferase	Toxin
GTF75_RS16035	401000..401257	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
GTF75_RS16040	401245..401547	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GTF75_RS16045	401781..402698	+	918	WP_000186333.1	alpha/beta hydrolase	-
GTF75_RS16050	402855..403244	+	390	WP_001081302.1	hypothetical protein	-
GTF75_RS16055	403380..403589	+	210	Protein_430	GNAT family N-acetyltransferase	-
GTF75_RS16060	403708..404145	+	438	WP_000503169.1	IS200/IS605-like element IS1004 family transposase	-
GTF75_RS16065	404209..404427	+	219	WP_071908318.1	GNAT family N-acetyltransferase	-
GTF75_RS16070	404602..405474	+	873	WP_000254716.1	DUF3800 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	305935..411136	105201
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	-	-	311015..410760	99745
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0	flank	IS/Tn	-	-	403708..404145	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (57-133)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 20171.46 Da
• Isoelectric Point: 9.1432

>T145877 WP_000118351.1 NZ_CP047302:400265-400798 [Vibrio cholerae O1 biovar El Tor]
VSWGKEFVELSKSKHDRDSFDCGEQELNTFIKTQAAKHMQAGISRTMVLPSAHPLLNQKFAICAFYSVAPSSISRETLPA
QLAKKLPRYPIPVFLLAQLAVHKEFHGSGLGKVSLIRALKYLWEVNHHMRAYAIVVDCLTDSAQAFYTKFGFEVLCEHNE
RIRMFLPMKTVEQLFNQ

Nucleotide

• Length: 534 bp

>T145877 NZ_CP062705:c3371432-3371058 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15714.41 Da
• Isoelectric Point: 7.6659

>AT145877 WP_001882332.1 NZ_CP047302:399843-400268 [Vibrio cholerae O1 biovar El Tor]
MLCLSLVVMRCQPLRRALYSPSPRKLSVRIECALSLAYNRSTDGMYPYAGGVMATARLDIRLDEEIKAKAEKASALLGLK
SLTEYVVRLMDEDATHVIEEHESIMVKDSVFDEFMAACNKVKAPNQALLEAVKFTEKSGIK

Nucleotide

• Length: 426 bp

>AT145877 NZ_CP062705:c3371853-3371479 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: Q9KM94