Detailed information of TA system TA058225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 402948..403563
• Replicon: chromosome
• Accession: NZ_CP013302
• Organism: Vibrio cholerae strain C5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: ASZ80_RS16475
• Protein ID: WP_001162670.1
• Coordinates: 402948..403235 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ASZ80_RS16480
• Protein ID: WP_001232701.1
• Coordinates: 403246..403563 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ80_RS20965	398584..398679	+	96	WP_001907607.1	DUF645 family protein	-
ASZ80_RS16435	398873..399190	-	318	WP_001180243.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ80_RS16440	399209..399451	-	243	WP_000557292.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ80_RS16445	399693..400202	+	510	WP_000405987.1	GNAT family N-acetyltransferase	-
ASZ80_RS16450	400259..400912	+	654	WP_000226874.1	hypothetical protein	-
ASZ80_RS16455	401222..401440	+	219	WP_001917086.1	DUF3709 domain-containing protein	-
ASZ80_RS16465	401843..402076	+	234	WP_032482776.1	DUF3709 domain-containing protein	-
ASZ80_RS16470	402501..402681	+	181	Protein_419	DUF645 family protein	-
ASZ80_RS16475	402948..403235	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ASZ80_RS16480	403246..403563	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
ASZ80_RS20970	403560..403670	+	111	WP_134814058.1	DUF3265 domain-containing protein	-
ASZ80_RS20975	403847..403972	+	126	WP_001944767.1	DUF645 family protein	-
ASZ80_RS20980	403988..404026	+	39	WP_106019119.1	hypothetical protein	-
ASZ80_RS16500	404250..404990	+	741	WP_000469836.1	PhzF family phenazine biosynthesis protein	-
ASZ80_RS16505	405195..406025	+	831	WP_000056616.1	abortive infection family protein	-
ASZ80_RS16510	406082..406564	+	483	WP_001895003.1	hypothetical protein	-
ASZ80_RS16520	407011..407886	+	876	WP_000259044.1	hypothetical protein	-
ASZ80_RS16525	408016..408471	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304694..469286	164592
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.005	inside	Integron	-	-	309774..468910	159136

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T58225 WP_001162670.1 NZ_CP013302:402948-403235 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T58225 NZ_CP013302:402948-403235 [Vibrio cholerae]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT58225 WP_001232701.1 NZ_CP013302:403246-403563 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT58225 NZ_CP013302:403246-403563 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36