74619

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 762297..763063 Replicon chromosome
Accession NZ_CP020407
Organism Vibrio cholerae strain FDAARGOS_223

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag A6J62_RS03485 Protein ID WP_000982260.1
Coordinates 762566..763063 (+) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9K2J6
Locus tag A6J62_RS03480 Protein ID WP_000246253.1
Coordinates 762297..762569 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A6J62_RS03435 757525..758442 - 918 WP_000186333.1 alpha/beta hydrolase -
A6J62_RS03440 758676..758978 - 303 WP_000229317.1 type II toxin-antitoxin system RelE/ParE family toxin -
A6J62_RS03445 758966..759223 - 258 WP_000861987.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
A6J62_RS03455 759425..759958 - 534 WP_000118351.1 GNAT family N-acetyltransferase -
A6J62_RS19635 759955..760380 - 426 WP_001882332.1 DUF1778 domain-containing protein -
A6J62_RS03465 760447..760818 - 372 WP_001164080.1 nucleotide pyrophosphohydrolase -
A6J62_RS03470 760959..761246 - 288 WP_000426470.1 hypothetical protein -
A6J62_RS03475 761398..762066 - 669 WP_000043871.1 hypothetical protein -
A6J62_RS03480 762297..762569 + 273 WP_000246253.1 DUF1778 domain-containing protein Antitoxin
A6J62_RS03485 762566..763063 + 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
A6J62_RS03495 763265..763417 - 153 WP_001884520.1 DUF645 family protein -
A6J62_RS03500 763739..764194 - 456 WP_001245327.1 GNAT family N-acetyltransferase -
A6J62_RS03505 764322..764609 - 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin -
A6J62_RS03510 764599..764850 - 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
A6J62_RS03515 765064..765477 - 414 WP_000049417.1 VOC family protein -
A6J62_RS03520 765552..765695 - 144 WP_071908341.1 DUF3265 domain-containing protein -
A6J62_RS03525 765665..766066 - 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin -
A6J62_RS03530 766066..766758 - 693 WP_001047169.1 GNAT family N-acetyltransferase -
A6J62_RS03535 766929..767201 - 273 Protein_667 CatB-related O-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 749087..881909 132822
inside Integron - - 749463..875103 125640
flank IS/Tn - - 767269..768249 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T74619 WP_000982260.1 NZ_CP020407:762566-763063 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T74619 NZ_CP020407:762566-763063 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9683.22 Da        Isoelectric Point: 6.2988

>AT74619 WP_000246253.1 NZ_CP020407:762297-762569 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT74619 NZ_CP020407:762297-762569 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
AlphaFold DB A0A0F2IC79

References