Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/RelE-MqsA |
| Location | 970665..971298 | Replicon | chromosome |
| Accession | NC_012667 | ||
| Organism | Vibrio cholerae MJ-1236 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | Q9KMA6 |
| Locus tag | VCD_RS19015 | Protein ID | WP_000843587.1 |
| Coordinates | 970966..971298 (-) | Length | 111 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | VCD_RS19010 | Protein ID | WP_000071008.1 |
| Coordinates | 970665..970979 (-) | Length | 105 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| VCD_RS18965 | 965719..966006 | - | 288 | WP_000221354.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| VCD_RS18970 | 965996..966247 | - | 252 | WP_001250179.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | - |
| VCD_RS18975 | 966461..966874 | - | 414 | WP_000049417.1 | VOC family protein | - |
| VCD_RS20710 | 966949..967092 | - | 144 | WP_071908341.1 | DUF3265 domain-containing protein | - |
| VCD_RS18980 | 967062..967463 | - | 402 | WP_000351248.1 | type II toxin-antitoxin system death-on-curing family toxin | - |
| VCD_RS18985 | 967463..968155 | - | 693 | WP_001047169.1 | GNAT family N-acetyltransferase | - |
| VCD_RS18990 | 968292..968598 | - | 307 | Protein_849 | CatB-related O-acetyltransferase | - |
| VCD_RS18995 | 968666..969646 | + | 981 | WP_000019370.1 | IS5-like element ISVch5 family transposase | - |
| VCD_RS19000 | 969771..969926 | - | 156 | WP_000751734.1 | hypothetical protein | - |
| VCD_RS19005 | 970094..970528 | - | 435 | WP_000256036.1 | GNAT family N-acetyltransferase | - |
| VCD_RS19010 | 970665..970979 | - | 315 | WP_000071008.1 | DNA-binding transcriptional regulator | Antitoxin |
| VCD_RS19015 | 970966..971298 | - | 333 | WP_000843587.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| VCD_RS20360 | 971639..971866 | - | 228 | WP_001894457.1 | DUF1289 domain-containing protein | - |
| VCD_RS20365 | 971815..971928 | - | 114 | WP_001889158.1 | hypothetical protein | - |
| VCD_RS20380 | 972094..972375 | - | 282 | WP_001894453.1 | DUF1289 domain-containing protein | - |
| VCD_RS21065 | 972324..972438 | - | 115 | Protein_858 | acetyltransferase | - |
| VCD_RS19025 | 972495..972872 | - | 378 | WP_000411109.1 | hypothetical protein | - |
| VCD_RS19030 | 973043..973285 | - | 243 | WP_000107461.1 | hypothetical protein | - |
| VCD_RS19035 | 973487..973873 | - | 387 | WP_000703163.1 | VOC family protein | - |
| VCD_RS20385 | 974082..974253 | - | 172 | Protein_862 | DUF645 family protein | - |
| VCD_RS19040 | 974571..974840 | - | 270 | WP_001198131.1 | hypothetical protein | - |
| VCD_RS20885 | 975200..975373 | - | 174 | WP_001882305.1 | DUF3709 domain-containing protein | - |
| VCD_RS21070 | 975654..975857 | - | 204 | WP_001911745.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | catB9 | - | 932253..1057432 | 125179 | |
| inside | Integron | - | - | 958376..1050596 | 92220 | ||
| flank | IS/Tn | - | - | 968666..969646 | 980 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 111 a.a. Molecular weight: 13005.95 Da Isoelectric Point: 9.9244
>T23786 WP_000843587.1 NC_012667:c971298-970966 [Vibrio cholerae MJ-1236]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS
Download Length: 333 bp
>T23786 NC_012667:c971298-970966 [Vibrio cholerae MJ-1236]
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGGCGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGGCGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA
Antitoxin
Download Length: 105 a.a. Molecular weight: 11696.20 Da Isoelectric Point: 6.7600
>AT23786 WP_000071008.1 NC_012667:c970979-970665 [Vibrio cholerae MJ-1236]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL
Download Length: 315 bp
>AT23786 NC_012667:c970979-970665 [Vibrio cholerae MJ-1236]
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCGCGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCGCGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|