Detailed information of TA system TA046759

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-GNAT
• Location: 119871..120964
• Replicon: chromosome
• Accession: NZ_CP007635
• Organism: Vibrio cholerae strain 2012EL-2176

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9KMA2
• Locus tag: EN18_RS14940
• Protein ID: WP_000351248.1
• Coordinates: 120563..120964 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: EN18_RS14935
• Protein ID: WP_001047169.1
• Coordinates: 119871..120563 (+)
• Length: 231 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EN18_RS14895	115154..115531	+	378	WP_000411109.1	hypothetical protein	-
EN18_RS21575	115588..115702	+	115	Protein_174	acetyltransferase	-
EN18_RS20695	115651..115932	+	282	WP_001894453.1	DUF1289 domain-containing protein	-
EN18_RS20710	116098..116211	+	114	WP_001889158.1	hypothetical protein	-
EN18_RS20715	116160..116387	+	228	WP_073426587.1	DUF1289 domain-containing protein	-
EN18_RS14905	116728..117060	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EN18_RS14910	117047..117361	+	315	WP_000071008.1	DNA-binding transcriptional regulator	-
EN18_RS14915	117498..117932	+	435	WP_000256036.1	GNAT family N-acetyltransferase	-
EN18_RS21395	118100..118255	+	156	WP_000751734.1	hypothetical protein	-
EN18_RS14925	118380..119360	-	981	WP_000019370.1	IS5-like element ISVch5 family transposase	-
EN18_RS14930	119428..119734	+	307	Protein_183	CatB-related O-acetyltransferase	-
EN18_RS14935	119871..120563	+	693	WP_001047169.1	GNAT family N-acetyltransferase	Antitoxin
EN18_RS14940	120563..120964	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
EN18_RS21220	120934..121077	+	144	WP_071908341.1	DUF3265 domain-containing protein	-
EN18_RS14945	121152..121565	+	414	WP_000049417.1	VOC family protein	-
EN18_RS14950	121779..122030	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
EN18_RS14955	122020..122307	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EN18_RS14960	122435..122890	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
EN18_RS14965	123212..123364	+	153	WP_001884520.1	DUF645 family protein	-
EN18_RS14970	123566..124063	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
EN18_RS14975	124060..124332	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
EN18_RS14980	124563..125231	+	669	WP_000043871.1	hypothetical protein	-
EN18_RS14985	125383..125670	+	288	WP_000426470.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	31037..137542	106505
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	-	-	36117..137166	101049
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000174, TA000175, TA000176, TA010030 416601..417581 0	flank	IS/Tn	-	-	118380..119360	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-132)

Antitoxin

• GNAT domain (36-153)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14608.71 Da
• Isoelectric Point: 4.0128

>T46759 WP_000351248.1 NZ_CP007635:120563-120964 [Vibrio cholerae]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Nucleotide

• Length: 402 bp

>T46759 NZ_CP007635:120563-120964 [Vibrio cholerae]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin

Protein

• Length: 231 a.a.
• Molecular weight: 26352.73 Da
• Isoelectric Point: 8.5633

>AT46759 WP_001047169.1 NZ_CP007635:119871-120563 [Vibrio cholerae]
MNLEEFQESDFDLLIKWIDSDELNYLWGCPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVMVVVIEFGGMRCQPLRRALCFLSRF
GAIIGLTFIDSRCDMNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 693 bp

>AT46759 NZ_CP007635:119871-120563 [Vibrio cholerae]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTTGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTATGGTTGTTGTGATTGAGTTTGGCGGTATGCGTTGTCAGCCCCTTAGGCGGGCGTTATGTTTTTTATCACGTTTT
GGGGCTATAATCGGCCTAACCTTTATTGATAGTAGGTGTGATATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGT
TGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGACTGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTA
AACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGGCTGATATGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q0PN34