89004

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RelE-RelB
Location 431250..431809 Replicon chromosome
Accession NZ_CP024868
Organism Vibrio cholerae strain A1552

Toxin (Protein)


Gene name relE Uniprot ID Q9K2M8
Locus tag VCA1552_RS16915 Protein ID WP_000578476.1
Coordinates 431250..431528 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q9L991
Locus tag VCA1552_RS16920 Protein ID WP_000381183.1
Coordinates 431525..431809 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VCA1552_RS16865 426550..426987 + 438 WP_000503169.1 IS200/IS605-like element IS1004 family transposase -
VCA1552_RS16870 427051..427269 + 219 WP_071908318.1 GNAT family N-acetyltransferase -
VCA1552_RS16875 427444..428316 + 873 WP_000254716.1 DUF3800 domain-containing protein -
VCA1552_RS16880 428466..429065 + 600 WP_001891245.1 glutathione S-transferase N-terminal domain-containing protein -
VCA1552_RS16885 429122..429226 + 105 WP_099607150.1 acetyltransferase -
VCA1552_RS16890 429257..429526 + 270 WP_001114075.1 DUF4160 domain-containing protein -
VCA1552_RS16895 429520..430041 + 522 WP_000921691.1 DUF2442 domain-containing protein -
VCA1552_RS16900 430340..430465 + 126 WP_001905700.1 DUF645 family protein -
VCA1552_RS16910 430716..431111 + 396 WP_001000867.1 hypothetical protein -
VCA1552_RS16915 431250..431528 - 279 WP_000578476.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
VCA1552_RS16920 431525..431809 - 285 WP_000381183.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
VCA1552_RS16925 432003..432518 + 516 WP_000343779.1 GNAT family N-acetyltransferase -
VCA1552_RS16935 432702..433115 + 414 WP_000049420.1 VOC family protein -
VCA1552_RS16950 433670..434847 + 1178 WP_086010879.1 IS3-like element ISVch4 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 304717..433978 129261
inside Integron - - 309797..433602 123805
flank IS/Tn - - 426550..426987 437


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(2-89)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-70)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10819.59 Da        Isoelectric Point: 5.1651

>T89004 WP_000578476.1 NZ_CP024868:c431528-431250 [Vibrio cholerae]
MIFWEEASLNDREKIFEFLYDFNPAAAKKTDELIEAKVENLLEQPLIGVQRDGIRGRLLIIPEISMIVSYWVDGSKIRIM
RVLHQKQKFPND

Download         Length: 279 bp

>T89004 NZ_CP024868:c431528-431250 [Vibrio cholerae]
ATGATTTTCTGGGAAGAAGCATCTCTCAATGATCGTGAGAAAATTTTCGAATTTCTCTACGACTTTAACCCAGCAGCGGC
TAAAAAAACGGATGAGCTCATAGAAGCCAAAGTCGAAAATTTGCTTGAGCAACCACTAATCGGTGTTCAGCGTGATGGCA
TTAGAGGCAGATTGCTTATTATCCCTGAGATATCAATGATTGTTTCGTATTGGGTTGATGGTTCTAAAATTCGAATAATG
CGTGTACTACATCAGAAACAAAAATTTCCAAACGACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10901.25 Da        Isoelectric Point: 8.0775

>AT89004 WP_000381183.1 NZ_CP024868:c431809-431525 [Vibrio cholerae]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKALSHDAWLTEQVNQAFEKFDSGKAVFIEHDIAKAR
MAERKAKIRNRGHA

Download         Length: 285 bp

>AT89004 NZ_CP024868:c431809-431525 [Vibrio cholerae]
ATGGATACTAGAATTCAATTTCGTGTAGACGAAGAAACAAAACGTTTAGCTCAACAAATGGCTGAAAGCCAAGGTCGAAC
TCTTAGCGATGCTTGCCGTGAACTCACTGAACAATTAGCTGAGCAGCAACGCAAGGCATTATCTCACGATGCATGGCTAA
CTGAACAAGTGAATCAAGCATTTGAGAAGTTCGACTCAGGAAAAGCAGTATTCATTGAACATGACATCGCCAAAGCACGA
ATGGCTGAACGTAAAGCTAAAATCCGAAATCGAGGCCACGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q9K2M8

Antitoxin

Source ID Structure
AlphaFold DB A0A067BL33

References