23721

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RelE-RelB
Location 407050..407609 Replicon chromosome II
Accession NC_012580
Organism Vibrio cholerae M66-2

Toxin (Protein)


Gene name relE Uniprot ID Q9K2M8
Locus tag VCM66_RS15435 Protein ID WP_000578476.1
Coordinates 407050..407328 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q9L991
Locus tag VCM66_RS15440 Protein ID WP_000381183.1
Coordinates 407325..407609 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VCM66_RS15400 402350..402787 + 438 WP_000503169.1 IS200/IS605-like element IS1004 family transposase -
VCM66_RS15405 402851..403069 + 219 WP_071908318.1 GNAT family N-acetyltransferase -
VCM66_RS15410 403244..404116 + 873 WP_000254716.1 DUF3800 domain-containing protein -
VCM66_RS15415 404266..404865 + 600 WP_001891245.1 glutathione S-transferase N-terminal domain-containing protein -
VCM66_RS19440 404922..405026 + 105 WP_099607150.1 acetyltransferase -
VCM66_RS15420 405057..405326 + 270 WP_001114075.1 DUF4160 domain-containing protein -
VCM66_RS15425 405320..405841 + 522 WP_000921691.1 DUF2442 domain-containing protein -
VCM66_RS19445 406140..406265 + 126 WP_001905700.1 DUF645 family protein -
VCM66_RS15430 406516..406911 + 396 WP_001000867.1 hypothetical protein -
VCM66_RS15435 407050..407328 - 279 WP_000578476.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
VCM66_RS15440 407325..407609 - 285 WP_000381183.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
VCM66_RS15445 407803..408318 + 516 WP_000343779.1 GNAT family N-acetyltransferase -
VCM66_RS15450 408502..408915 + 414 WP_000049420.1 VOC family protein -
VCM66_RS15460 409470..410647 + 1178 WP_086010879.1 IS3-like element ISVch4 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 305869..409778 103909
- inside Integron - - 310949..409402 98453
flank IS/Tn - - 402350..402787 437


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(2-89)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-70)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10819.59 Da        Isoelectric Point: 5.1651

>T23721 WP_000578476.1 NC_012580:c407328-407050 [Vibrio cholerae M66-2]
MIFWEEASLNDREKIFEFLYDFNPAAAKKTDELIEAKVENLLEQPLIGVQRDGIRGRLLIIPEISMIVSYWVDGSKIRIM
RVLHQKQKFPND

Download         Length: 279 bp

>T23721 NC_012580:c407328-407050 [Vibrio cholerae M66-2]
ATGATTTTCTGGGAAGAAGCATCTCTCAATGATCGTGAGAAAATTTTCGAATTTCTCTACGACTTTAACCCAGCAGCGGC
TAAAAAAACGGATGAGCTCATAGAAGCCAAAGTCGAAAATTTGCTTGAGCAACCACTAATCGGTGTTCAGCGTGATGGCA
TTAGAGGCAGATTGCTTATTATCCCTGAGATATCAATGATTGTTTCGTATTGGGTTGATGGTTCTAAAATTCGAATAATG
CGTGTACTACATCAGAAACAAAAATTTCCAAACGACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10901.25 Da        Isoelectric Point: 8.0775

>AT23721 WP_000381183.1 NC_012580:c407609-407325 [Vibrio cholerae M66-2]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKALSHDAWLTEQVNQAFEKFDSGKAVFIEHDIAKAR
MAERKAKIRNRGHA

Download         Length: 285 bp

>AT23721 NC_012580:c407609-407325 [Vibrio cholerae M66-2]
ATGGATACTAGAATTCAATTTCGTGTAGACGAAGAAACAAAACGTTTAGCTCAACAAATGGCTGAAAGCCAAGGTCGAAC
TCTTAGCGATGCTTGCCGTGAACTCACTGAACAATTAGCTGAGCAGCAACGCAAGGCATTATCTCACGATGCATGGCTAA
CTGAACAAGTGAATCAAGCATTTGAGAAGTTCGACTCAGGAAAAGCAGTATTCATTGAACATGACATCGCCAAAGCACGA
ATGGCTGAACGTAAAGCTAAAATCCGAAATCGAGGCCACGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q9K2M8

Antitoxin

Source ID Structure
AlphaFold DB A0A067BL33

References