Detailed information of TA system
Overview
TA module
| Type | I | Classification (family/domain) | hok-sok/- |
| Location | 346202..346459 | Replicon | chromosome |
| Accession | NZ_CP020543 | ||
| Organism | Escherichia coli C | ||
| T1TAdb ID | TA07126 | ||
Toxin (Protein)
| Gene name | hokA | Uniprot ID | V0YDF1 |
| Locus tag | B6N50_RS01665 | Protein ID | WP_001135738.1 |
| Coordinates | 346202..346354 (-) | Length | 51 a.a. |
Antitoxin (RNA)
| Gene name | sokA | ||
| Locus tag | - | ||
| Coordinates | 346405..346459 (+) |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| B6N50_RS01640 (B6N50_01640) | 342301..342960 | + | 660 | WP_000747625.1 | OmpA family lipoprotein | - |
| B6N50_RS01645 (B6N50_01645) | 343064..344038 | + | 975 | WP_000805011.1 | glyoxylate/hydroxypyruvate reductase GhrB | - |
| B6N50_RS01650 (B6N50_01650) | 344088..344798 | - | 711 | WP_000190516.1 | DUF3053 domain-containing protein | - |
| B6N50_RS01655 (B6N50_01655) | 345232..345522 | + | 291 | WP_000455798.1 | HTH-type transcriptional regulator | - |
| B6N50_RS01660 (B6N50_01660) | 345803..346015 | + | 213 | WP_000014594.1 | RNA chaperone/antiterminator CspA | - |
| B6N50_RS01665 (B6N50_01665) | 346202..346354 | - | 153 | WP_001135738.1 | type I toxin-antitoxin system toxin HokA | Toxin |
| - | 346405..346459 | + | 55 | - | - | Antitoxin |
| B6N50_RS01670 (B6N50_01670) | 346677..348746 | - | 2070 | WP_081508762.1 | glycine--tRNA ligase subunit beta | - |
| B6N50_RS01675 (B6N50_01675) | 348756..349667 | - | 912 | WP_001168544.1 | glycine--tRNA ligase subunit alpha | - |
| B6N50_RS01680 (B6N50_01680) | 349762..350061 | - | 300 | WP_000980111.1 | YsaB family lipoprotein | - |
| B6N50_RS01685 (B6N50_01685) | 350236..351231 | + | 996 | WP_047402597.1 | O-acetyltransferase WecH | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 51 a.a. Molecular weight: 5912.14 Da Isoelectric Point: 7.7173
>T10208 WP_001135738.1 NZ_CP020543:c346354-346202 [Escherichia coli C]
MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFLACNLKE
MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFLACNLKE
Download Length: 153 bp
>T10208 NZ_CP020543:c346354-346202 [Escherichia coli C]
ATGCCGCAGAAATATAGATTACTTTCTTTAATAGTGATTTGTTTCACGCTTTTATTTTTCACCTGGATGATAAGAGATTC
ACTGTGTGAATTGCATATTAAACAGGGGAGTTATGAGCTGGCGGCATTTTTAGCCTGCAATTTAAAAGAGTAA
ATGCCGCAGAAATATAGATTACTTTCTTTAATAGTGATTTGTTTCACGCTTTTATTTTTCACCTGGATGATAAGAGATTC
ACTGTGTGAATTGCATATTAAACAGGGGAGTTATGAGCTGGCGGCATTTTTAGCCTGCAATTTAAAAGAGTAA
Antitoxin
Download Length: 55 bp
>AT10208 NZ_CP020543:346405-346459 [Escherichia coli C]
GTTCAGGCATAGGGTAGCCTCACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTG
GTTCAGGCATAGGGTAGCCTCACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10210 | Hafnia alvei |
68.182 |
88 |
0.6 |
| T6370 | Escherichia coli |
54 |
100 |
0.54 |
| T10124 | Erwinia amylovora ATCC 49946 |
53.846 |
81.25 |
0.437 |
| T6324 | Escherichia coli |
40 |
90 |
0.36 |
| T6363 | uncultured bacterium |
40 |
90 |
0.36 |
| T10039 | Escherichia coli O157:H7 str. Sakai |
42.857 |
84 |
0.36 |
| T10209 | Escherichia coli strain ECOR24 |
41.86 |
86 |
0.36 |
| T6369 | Escherichia coli K-12 |
40.476 |
85.714 |
0.347 |
| T10035 | Vibrio cholerae O1 biovar El Tor str. N16961 |
37.5 |
80 |
0.3 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) K Pedersen et al. (1999) Multiple hok genes on the chromosome of Escherichia coli. Molecular Microbiology 32(5):1090-102. [PubMed:10361310]