6370

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 55829..56093 Replicon plasmid pIFM3804
Accession NC_023329
Organism Escherichia coli

Toxin (Protein)


Gene name pndA Uniprot ID U9Y6M3
Locus tag D616_p112058 Protein ID WP_001331364.1
Coordinates 55941..56093 (+) Length 51 a.a.

Antitoxin (RNA)


Gene name pndB
Locus tag D616_s112002
Coordinates 55829..55891 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTE28_RS00320 (D616_p112062) 51068..53359 - 2292 WP_001289276.1 F-type conjugative transfer protein TrbC -
HTE28_RS00325 (D616_p112061) 53352..54422 - 1071 WP_000151582.1 IncI1-type conjugal transfer protein TrbB -
HTE28_RS00330 (D616_p112060) 54441..55649 - 1209 WP_000121273.1 IncI1-type conjugal transfer protein TrbA -
- 55829..55891 - 63 - - Antitoxin
HTE28_RS00335 (D616_p112058) 55941..56093 + 153 WP_001331364.1 Hok/Gef family protein Toxin
HTE28_RS00340 (D616_p112057) 56165..56416 - 252 WP_001291964.1 hypothetical protein -
HTE28_RS00620 56914..57009 + 96 WP_001303310.1 DinQ-like type I toxin DqlB -
HTE28_RS00345 (D616_p112055) 57074..57250 - 177 WP_001054904.1 hypothetical protein -
HTE28_RS00350 (D616_p112053) 57641..57850 + 210 WP_000062603.1 HEAT repeat domain-containing protein -
HTE28_RS00355 (D616_p112052) 57922..58404 - 483 WP_001672078.1 plasmid IncI1-type surface exclusion protein ExcA -
HTE28_RS00360 (D616_p112051) 58645..60813 - 2169 WP_021265679.1 DotA/TraY family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid sul2 / floR / blaCTX-M-1 - 1..104399 104399


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(7-46)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5775.13 Da        Isoelectric Point: 8.7948

>T6370 WP_001331364.1 NC_023329:55941-56093 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Download         Length: 153 bp

>T6370 NC_023329:55941-56093 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 63 bp

>AT6370 NC_023329:c55891-55829 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T10208 Escherichia coli C

54

100

0.54
T10210 Hafnia alvei

52

100

0.52
T6369 Escherichia coli K-12

45.455

89.796

0.408
T10124 Erwinia amylovora ATCC 49946

39.286

87.5

0.344
T10039 Escherichia coli O157:H7 str. Sakai

33.333

96

0.32
T6363 uncultured bacterium

31.915

94

0.3
T6324 Escherichia coli

31.915

94

0.3

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5W9M382

Antitoxin

Download structure file

References

(1) A K Nielsen et al. (1991) The rifampicin-inducible genes srnB from F and pnd from R483 are regulated by antisense RNAs and mediate plasmid maintenance by killing of plasmid-free segregants. Molecular Microbiology 5(8):1961-73. [PubMed:1722558] experimental

(2) Régine Brielle et al. (2016) Linking bacterial type I toxins with their actions. Current Opinion in Microbiology 30:114-121. [PubMed:26874964]


experimental experimental literature