177243

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 15193..15740 Replicon plasmid unnamed476
Accession NZ_CP062903
Organism Escherichia coli O152:H23 strain Res13-Lact-ER01-35

Toxin (Protein)


Gene name parE Uniprot ID W0LWB3
Locus tag IMP37_RS23585 Protein ID WP_000970007.1
Coordinates 15459..15740 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag IMP37_RS23580 Protein ID WP_000079941.1
Coordinates 15193..15462 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP37_RS23550 10867..11622 + 756 Protein_11 IS110-like element IS5075 family transposase -
IMP37_RS23555 11804..11980 - 177 WP_000954592.1 Arm DNA-binding domain-containing protein -
IMP37_RS23560 12310..13125 + 816 WP_001043260.1 sulfonamide-resistant dihydropteroate synthase Sul2 -
IMP37_RS23565 13212..13454 + 243 Protein_14 phosphoglucosamine mutase -
IMP37_RS23570 13408..13659 - 252 WP_001445143.1 hypothetical protein -
IMP37_RS23575 13690..14826 - 1137 WP_023140202.1 IS91 family transposase -
IMP37_RS23580 15193..15462 + 270 WP_000079941.1 hypothetical protein Antitoxin
IMP37_RS23585 15459..15740 + 282 WP_000970007.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IMP37_RS23590 15780..16628 + 849 WP_021265674.1 3'-5' exonuclease -
IMP37_RS23595 16745..17218 + 474 WP_001334658.1 hypothetical protein -
IMP37_RS23600 17695..17961 - 267 WP_000955263.1 hypothetical protein -
IMP37_RS23605 17961..18455 - 495 WP_001459505.1 hypothetical protein -
IMP37_RS23610 18511..19113 - 603 WP_000517695.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid aadA5 / sul2 / blaCTX-M-1 - 1..112293 112293


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10892.63 Da        Isoelectric Point: 9.7617

>T177243 WP_000970007.1 NZ_CP062903:15459-15740 [Escherichia coli O152:H23]
MMEIFWTMLASQDRKRIRKYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T177243 NZ_CP083366:c5174505-5174158 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGCGGCAGTGATCTTGGTTCTGCGACAGAAGCAGAGTGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT177243 WP_000079941.1 NZ_CP062903:15193-15462 [Escherichia coli O152:H23]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT177243 NZ_CP083366:c5174766-5174515 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGACTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G1GDN5

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References