96767

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 14143..14690 Replicon plasmid pCFS3313-2
Accession NZ_CP026941
Organism Escherichia coli strain CFS3313

Toxin (Protein)


Gene name parE Uniprot ID W0LWB3
Locus tag C5F74_RS24485 Protein ID WP_000970007.1
Coordinates 14409..14690 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag C5F74_RS24480 Protein ID WP_000079941.1
Coordinates 14143..14412 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5F74_RS24445 9817..10572 + 756 Protein_12 IS110-like element IS5075 family transposase -
C5F74_RS24450 10754..10930 - 177 WP_000954592.1 Arm DNA-binding domain-containing protein -
C5F74_RS24455 11159..11236 - 78 Protein_14 transcriptional regulator -
C5F74_RS24460 11260..12075 + 816 WP_001043260.1 sulfonamide-resistant dihydropteroate synthase Sul2 -
C5F74_RS24465 12162..12464 + 303 WP_000251875.1 phosphoglucosamine mutase -
C5F74_RS24470 12358..12609 - 252 WP_001445143.1 hypothetical protein -
C5F74_RS24475 12640..13776 - 1137 WP_023140202.1 IS91 family transposase -
C5F74_RS24480 14143..14412 + 270 WP_000079941.1 hypothetical protein Antitoxin
C5F74_RS24485 14409..14690 + 282 WP_000970007.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C5F74_RS24490 14730..15578 + 849 WP_021265674.1 3'-5' exonuclease -
C5F74_RS24495 15695..16168 + 474 WP_001334658.1 hypothetical protein -
C5F74_RS24500 16911..17405 - 495 WP_001459505.1 hypothetical protein -
C5F74_RS24505 17461..18063 - 603 WP_000517695.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid tet(A) / sul2 / blaCTX-M-1 - 1..111813 111813
- inside IScluster/Tn tet(A) / sul2 - 2216..19654 17438


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10892.63 Da        Isoelectric Point: 9.7617

>T96767 WP_000970007.1 NZ_CP026941:14409-14690 [Escherichia coli]
MMEIFWTMLASQDRKRIRKYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T96767 NZ_CP026941:14409-14690 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTAAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT96767 WP_000079941.1 NZ_CP026941:14143-14412 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT96767 NZ_CP026941:14143-14412 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATTCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G1GDN5

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References