Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 9320..9867 Replicon plasmid PCN061p5
Accession NZ_CP006641
Organism Escherichia coli PCN061

Toxin (Protein)


Gene name parE Uniprot ID W0LWB3
Locus tag PCN061_RS23530 Protein ID WP_000970007.1
Coordinates 9586..9867 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag PCN061_RS23525 Protein ID WP_000079941.1
Coordinates 9320..9589 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PCN061_RS23505 5138..5443 - 306 WP_001255015.1 LysR family transcriptional regulator -
PCN061_RS23510 5471..6685 - 1215 WP_000214121.1 chloramphenicol/florfenicol efflux MFS transporter FloR -
PCN061_RS29500 6902..7348 - 447 Protein_7 DUF3363 domain-containing protein -
PCN061_RS23520 7817..8962 - 1146 Protein_8 IS91-like element ISVsa3 family transposase -
PCN061_RS23525 9320..9589 + 270 WP_000079941.1 hypothetical protein Antitoxin
PCN061_RS23530 9586..9867 + 282 WP_000970007.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PCN061_RS23535 9907..10755 + 849 WP_021265674.1 3'-5' exonuclease -
PCN061_RS23540 10872..11345 + 474 WP_001334658.1 hypothetical protein -
PCN061_RS29505 11822..12088 - 267 WP_000955263.1 hypothetical protein -
PCN061_RS23550 12088..12582 - 495 WP_001459505.1 hypothetical protein -
PCN061_RS23555 12638..13240 - 603 WP_000517695.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid floR - 1..103644 103644


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10892.63 Da        Isoelectric Point: 9.7617

>T45354 WP_000970007.1 NZ_CP006641:9586-9867 [Escherichia coli PCN061]
MMEIFWTMLASQDRKRIRKYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T45354 NZ_CP006641:9586-9867 [Escherichia coli PCN061]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTAAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT45354 WP_000079941.1 NZ_CP006641:9320-9589 [Escherichia coli PCN061]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT45354 NZ_CP006641:9320-9589 [Escherichia coli PCN061]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATTCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G1GDN5

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References