Detailed information of TA system
Overview
TA module
| Type | I | Classification (family/domain) | hok-sok/- |
| Location | 192..462 | Replicon | plasmid R1 |
| Accession | NG_034386 | ||
| Organism | Escherichia coli | ||
| T1TAdb ID | TA07123 | ||
Toxin (Protein)
| Gene name | hok | Uniprot ID | - |
| Locus tag | hok | Protein ID | YP_009073885.1 |
| Coordinates | 304..462 (+) | Length | 53 a.a. |
Antitoxin (RNA)
| Gene name | sok | ||
| Locus tag | sok | ||
| Coordinates | 192..255 (-) |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Locus_0 | 36..260 | - | 225 | YP_009073884.1 | - | - |
| - | 192..255 | - | 64 | - | - | Antitoxin |
| Locus_1 | 304..462 | + | 159 | YP_009073885.1 | - | Toxin |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..580 | 580 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 53 a.a. Molecular weight: 6082.32 Da Isoelectric Point: 9.2584
>T6324 YP_009073885.1 NG_034386:304-462 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK
Download Length: 159 bp
>T6324 NG_034386:304-462 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG
Antitoxin
Download Length: 64 bp
>AT6324 NG_034386:c255-192 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T6363 | uncultured bacterium |
100 |
100 |
1 |
| T10124 | Erwinia amylovora ATCC 49946 |
93.75 |
100 |
0.938 |
| T10039 | Escherichia coli O157:H7 str. Sakai |
44.231 |
100 |
0.451 |
| T10210 | Hafnia alvei |
47.917 |
92.308 |
0.442 |
| T10209 | Escherichia coli strain ECOR24 |
38.298 |
94 |
0.36 |
| T10208 | Escherichia coli C |
40 |
90 |
0.36 |
| T6369 | Escherichia coli K-12 |
39.024 |
83.673 |
0.327 |
| T6370 | Escherichia coli |
31.915 |
94 |
0.3 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) K Gerdes et al. (1990) Mechanism of post-segregational killing by the hok/sok system of plasmid R1: sok antisense RNA regulates formation of a hok mRNA species correlated with killing of plasmid-free cells. Molecular Microbiology 4(11):1807-18. [PubMed:1707122]