Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 21508..21777 Replicon plasmid pRSB225
Accession NC_025100
Organism uncultured bacterium

Toxin (Protein)


Gene name hok Uniprot ID -
Locus tag pRSB225_34 Protein ID YP_009065991.1
Coordinates 21619..21777 (+) Length 53 a.a.

Antitoxin (RNA)


Gene name sok
Locus tag pRSB225_32
Coordinates 21508..21573 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
pRSB225_27 16729..18687 + 1959 YP_009065986.1 ParB-like partition protein -
pRSB225_28 18742..19176 + 435 YP_009065987.1 plasmid SOS inhibition protein B -
pRSB225_29 19173..19892 + 720 YP_009065988.1 plasmid SOS inhibition protein A -
pRSB225_30 20052..20573 + 522 YP_009065989.1 transposase InsJ -
pRSB225_31 20570..21421 + 852 YP_009065990.1 transposase InsK -
- 21508..21573 - 66 - - Antitoxin
pRSB225_34 21619..21777 + 159 YP_009065991.1 small toxic polypeptide Toxin
pRSB225_35 21902..22057 + 156 YP_009065992.1 hypothetical protein -
pRSB225_36 22220..22555 + 336 YP_009065993.1 hypothetical protein -
pRSB225_37 22699..22986 + 288 YP_009065994.1 hypothetical protein -
pRSB225_38 23105..23926 + 822 YP_009065995.1 hypothetical protein -
pRSB225_39 24046..24195 + 150 YP_009065996.1 hypothetical protein -
pRSB225_40 24221..24811 - 591 YP_009065997.1 lytic transglycosylase -
pRSB225_41 25154..25537 + 384 YP_009065998.1 conjugal transfer protein TraM -
pRSB225_42 25671..26348 + 678 YP_009065999.1 conjugal transfer protein TraJ -
pRSB225_43 26436..26663 + 228 YP_009066000.1 conjugal transfer protein TraY -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / mph(A) / dfrA14 / catA1 / tet(B) / sitABCD iutA / iucD / iucC / iucB / iucA 1..164550 164550


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(8-48)


Sequences

Toxin        


Download         Length: 53 a.a.        Molecular weight: 6082.32 Da        Isoelectric Point: 9.2584

>T6363 YP_009065991.1 NC_025100:21619-21777 [uncultured bacterium]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Download         Length: 159 bp

>T6363 NC_025100:21619-21777 [uncultured bacterium]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTTACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin


Download         Length: 66 bp

>AT6363 NC_025100:c21573-21508 [uncultured bacterium]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T6324 Escherichia coli

100

100

1
T10124 Erwinia amylovora ATCC 49946

93.75

100

0.938
T10039 Escherichia coli O157:H7 str. Sakai

44.231

100

0.451
T10210 Hafnia alvei

47.917

92.308

0.442
T10209 Escherichia coli strain ECOR24

38.298

94

0.36
T10208 Escherichia coli C

40

90

0.36
T6369 Escherichia coli K-12

39.024

83.673

0.327
T6370 Escherichia coli

31.915

94

0.3

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Download structure file

References

(1) Daniel Wibberg et al. (2013) The IncF plasmid pRSB225 isolated from a municipal wastewater treatment plant's on-site preflooder combining antibiotic resistance and putative virulence functions is highly related to virulence plasmids identified in pathogenic E. coli isolates. Plasmid 69(2):127-37. [PubMed:23212116]