Detailed information of TA system
Overview
TA module
| Type | I | Classification (family/domain) | hok-sok/- |
| Location | 1305419..1305644 | Replicon | chromosome |
| Accession | NC_002695 | ||
| Organism | Escherichia coli O157:H7 str. Sakai | ||
Toxin (Protein)
| Gene name | hokW | Uniprot ID | - |
| Locus tag | - | Protein ID | - |
| Coordinates | 1305419..1305574 (-) | Length | 52 a.a. |
Antitoxin (RNA)
| Gene name | sokW | ||
| Locus tag | - | ||
| Coordinates | 1305586..1305644 (+) |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ECs_1244 | 1304955..1305299 | - | 345 | NP_309271.1 | hypothetical protein | - |
| - | 1305419..1305574 | - | 156 | - | - | Toxin |
| - | 1305586..1305644 | + | 59 | - | - | Antitoxin |
| ECs_1246 | 1305865..1306260 | - | 396 | NP_309273.1 | hypothetical protein | - |
| ECs_1247 | 1306260..1306919 | - | 660 | NP_309274.1 | hypothetical protein | - |
| ECs_1248 | 1306940..1307158 | - | 219 | NP_309275.1 | hypothetical protein | - |
| ECs_1249 | 1307145..1307429 | - | 285 | NP_309276.1 | hypothetical protein | - |
| ECs_1250 | 1307426..1307647 | - | 222 | NP_309277.1 | hypothetical protein | - |
| ECs_1251 | 1307695..1308324 | - | 630 | NP_309278.1 | phage antirepressor | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | Prophage | - | stx2A / stx2B | 1246182..1310801 | 64619 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 52 a.a. Molecular weight: 5767.95 Da Isoelectric Point: 7.7942
>T10039 - NC_002695:c1305574-1305419 [Escherichia coli O157:H7 str. Sakai]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK
Download Length: 156 bp
>T10039 NC_002695:c1305574-1305419 [Escherichia coli O157:H7 str. Sakai]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA
Antitoxin
Download Length: 59 bp
>AT10039 NC_002695:1305586-1305644 [Escherichia coli O157:H7 str. Sakai]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10209 | Escherichia coli strain ECOR24 |
80 |
100 |
0.8 |
| T10124 | Erwinia amylovora ATCC 49946 |
55.172 |
90.625 |
0.5 |
| T6324 | Escherichia coli |
44.231 |
100 |
0.451 |
| T6363 | uncultured bacterium |
44.231 |
100 |
0.451 |
| T10210 | Hafnia alvei |
42.857 |
96.078 |
0.412 |
| T6369 | Escherichia coli K-12 |
42.553 |
95.918 |
0.408 |
| T10208 | Escherichia coli C |
42.857 |
84 |
0.36 |
| T6370 | Escherichia coli |
33.333 |
96 |
0.32 |
| T10211 | E.coli NADPH-sulfite reductase flavoprotein component (cysJ) NADPH-sulfite reductase hemoprotein component (cysI) and 3' |
34.091 |
89.796 |
0.306 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Kosuke Takada et al. (2021) The hokW-sokW Locus Encodes a Type I Toxin-Antitoxin System That Facilitates the Release of Lysogenic Sp5 Phage in Enterohemorrhagic Escherichia coli O157. Toxins 13(11):796. [PubMed:34822580]