6369

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 2821..3075 Replicon plasmid F
Accession NC_002483
Organism Escherichia coli K-12

Toxin (Protein)


Gene name srnB Uniprot ID G9G1E3
Locus tag D616_p97086 Protein ID WP_001312851.1
Coordinates 2926..3075 (+) Length 50 a.a.

Antitoxin (RNA)


Gene name srnC
Locus tag D616_s97001
Coordinates 2821..2882 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXF02_RS00010 (D616_p97046) 66..1228 + 1163 WP_085947771.1 IS3-like element IS3 family transposase -
HXF02_RS00015 1259..1426 + 168 Protein_1 ProQ/FINO family protein -
HXF02_RS00020 1561..1749 + 189 WP_001345829.1 hypothetical protein -
HXF02_RS00515 1947..2033 + 87 Protein_3 endonuclease -
HXF02_RS00025 2214..2531 - 318 WP_250911031.1 hypothetical protein -
- 2821..2882 - 62 - - Antitoxin
HXF02_RS00030 (D616_p97087) 2926..3075 + 150 WP_001312851.1 Hok/Gef family protein Toxin
HXF02_RS00035 (D616_p97095) 3359..3616 + 258 WP_000083834.1 replication regulatory protein RepA -
HXF02_RS00040 (D616_p97091) 3850..3924 + 75 WP_001442103.1 RepA leader peptide Tap -
HXF02_RS00535 3962..4084 - 123 WP_289845690.1 hypothetical protein -
HXF02_RS00045 (D616_p97081) 4303..6399 - 2097 WP_000422420.1 hypothetical protein -
HXF02_RS00050 (D616_p97083) 6415..6966 - 552 WP_001235704.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid - - 1..99159 99159


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(4-45)


Sequences

Toxin        


Download         Length: 50 a.a.        Molecular weight: 5542.67 Da        Isoelectric Point: 8.7678

>T6369 WP_001312851.1 NC_002483:2926-3075 [Escherichia coli K-12]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Download         Length: 150 bp

>T6369 NC_002483:2926-3075 [Escherichia coli K-12]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCTACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 62 bp

>AT6369 NC_002483:c2882-2821 [Escherichia coli K-12]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T6370 Escherichia coli

45.455

89.796

0.408
T10039 Escherichia coli O157:H7 str. Sakai

42.553

95.918

0.408
T10210 Hafnia alvei

43.478

93.878

0.408
T10209 Escherichia coli strain ECOR24

36.957

93.878

0.347
T10208 Escherichia coli C

40.476

85.714

0.347
T10211 E.coli NADPH-sulfite reductase flavoprotein component (cysJ) NADPH-sulfite reductase hemoprotein component (cysI) and 3'

33.333

100

0.347
T10124 Erwinia amylovora ATCC 49946

35.484

96.875

0.344
T6363 uncultured bacterium

39.024

83.673

0.327
T6324 Escherichia coli

39.024

83.673

0.327

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829C8K5

Antitoxin

Download structure file

References

(1) A K Nielsen et al. (1991) The rifampicin-inducible genes srnB from F and pnd from R483 are regulated by antisense RNAs and mediate plasmid maintenance by killing of plasmid-free segregants. Molecular Microbiology 5(8):1961-73. [PubMed:1722558] experimental

(2) Régine Brielle et al. (2016) Linking bacterial type I toxins with their actions. Current Opinion in Microbiology 30:114-121. [PubMed:26874964]


experimental experimental literature