Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 45449..45974 Replicon plasmid pF
Accession NZ_CP076044
Organism Escherichia coli strain Stbl4

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag ITN70_RS21460 Protein ID WP_001159868.1
Coordinates 45669..45974 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag ITN70_RS21455 Protein ID WP_000813634.1
Coordinates 45449..45667 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ITN70_RS21440 (40605) 40605..41504 + 900 WP_000963206.1 nucleotide-binding protein -
ITN70_RS21445 (41554) 41554..43779 - 2226 WP_000698737.1 P-loop NTPase fold protein -
ITN70_RS21450 (43781) 43781..44869 - 1089 WP_000952217.1 transcriptional repressor PifC -
ITN70_RS21455 (45449) 45449..45667 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ITN70_RS21460 (45669) 45669..45974 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
ITN70_RS21465 (45975) 45975..46781 + 807 WP_000016982.1 site-specific integrase -
ITN70_RS21470 (47555) 47555..48310 + 756 WP_000852146.1 replication initiation protein RepE -
ITN70_RS21475 (48898) 48898..50064 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid - - 1..98268 98268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T204603 WP_001159868.1 NZ_CP076044:45669-45974 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T204603 NZ_CP101125:6026272-6026637 [Pseudomonas nunensis]
ATGAATTGGGATATTGAATACACCGACGAGTTCGGCGACTGGTGGACGGATCTGAGCGAAAGCGAGCAGAGTTCTGTCGC
CGCGAGCGTAAAACTACTCGGGCAGTTCGGCCCCGGTTTACGCTTTCCTCATAGCAGTGGAATCAATGGATCGCGGCACG
GCCATCTTCGTGAATTACGCGTTCAGCACGCAGGTCGCCCTTATCGCATCTTGTATGCATTCGATCCAAAACGTTGTGCG
TTGCTATTGATAGGAGGAGACAAAACCGGTCAGGATCGATGGTATGAAGTCAACGTACCGTTGGCCGATCAGCTGTACGA
TGAACATCTCGACACATTGCGCAAAGAGGGTCACAACGATGGCTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT204603 WP_000813634.1 NZ_CP076044:45449-45667 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT204603 NZ_CP101125:6026630-6026935 [Pseudomonas nunensis]
ATGGCTAAGAAATTTGCCGAACTCGAAGCGCGGATGACACCCGAATCCCGGGCACTCGCCGATTCGATTTATGAACAACA
CATCAAGGAAATGCCGCTGCACGAATTGCGTCAGGCCAAAGCGCTGAGTCAAGCCACGCTTGCCGAAACACTTCATATCA
ACCAGGCCGCCATTTCTAAAATGGAGCGCCGCACGGATATGTACATCAGCACCTTGCGCAACTACATCCGCGCTATGGGC
GGTGAGCTTGAAATCATTGCGACGTTTCCAGACGGCCAAATCAAGATCGAAAATTTTGCTGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References