20275

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 62713..63134 Replicon plasmid F
Accession NC_002483
Organism Escherichia coli K-12

Toxin (Protein)


Gene name hok Uniprot ID -
Locus tag HXF02_RS00305 Protein ID WP_096937776.1
Coordinates 63009..63134 (+) Length 42 a.a.

Antitoxin (RNA)


Gene name srnC
Locus tag -
Coordinates 62713..62911 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXF02_RS00275 57807..58163 - 357 WP_000388237.1 hypothetical protein -
HXF02_RS00280 (D616_p97085) 58643..59182 + 540 WP_000290801.1 single-stranded DNA-binding protein -
HXF02_RS00285 (D616_p97006) 59239..59472 + 234 WP_000005990.1 DUF905 family protein -
HXF02_RS00290 (D616_p97005) 59538..61496 + 1959 WP_000117173.1 ParB/RepB/Spo0J family partition protein -
HXF02_RS00295 (D616_p97097) 61551..61985 + 435 WP_010892539.1 conjugation system SOS inhibitor PsiB -
HXF02_RS00300 (D616_p97098) 61982..62744 + 763 Protein_60 plasmid SOS inhibition protein A -
- 62713..62911 - 199 - - Antitoxin
HXF02_RS00530 62918..63067 + 150 Protein_61 DUF5431 family protein -
HXF02_RS00305 (D616_p97103) 63009..63134 + 126 WP_096937776.1 type I toxin-antitoxin system Hok family toxin Toxin
HXF02_RS00310 (D616_p97004) 63435..63732 - 298 Protein_63 hypothetical protein -
HXF02_RS00320 (D616_p97003) 64032..64319 + 288 WP_000107542.1 hypothetical protein -
HXF02_RS00325 (D616_p97002) 64438..65259 + 822 WP_001234417.1 DUF932 domain-containing protein -
HXF02_RS00330 (D616_p97001) 65556..66158 - 603 WP_000243701.1 transglycosylase SLT domain-containing protein -
HXF02_RS00335 (D616_p97066) 66479..66862 + 384 WP_001151527.1 conjugal transfer relaxosome DNA-binding protein TraM -
HXF02_RS00340 (D616_p97072) 67049..67738 + 690 WP_000283385.1 conjugal transfer transcriptional regulator TraJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid - - 1..99159 99159


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(2-37)


Sequences

Toxin        


Download         Length: 42 a.a.        Molecular weight: 4806.72 Da        Isoelectric Point: 8.4890

>T20275 WP_096937776.1 NC_002483:63009-63134 [Escherichia coli K-12]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Download         Length: 126 bp

>T20275 NC_002483:63009-63134 [Escherichia coli K-12]
GTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACGAAAATCGCTGTGCGAGATTCGTTACAGAGACGG
ATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin


Download         Length: 199 bp

>AT20275 NC_002483:c62911-62713 [Escherichia coli K-12]
GCCTCGTGGGTTAATGAAAAATTAACTACGGGGCTTTTGTCCTTCTGCCACACAACACGGCAACAAACCACCTTCACGTC
ATGAGGCAGAAAGCCTCAAGCGCCGGGCACATCATAGCCCATATCCCTGCACGCTGACCACACTCACTTTTCCTGAAAAT
AACCCGCTCATTCAGGCTGTTTACGGGTAATCTGTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Download structure file

References