Detailed information of TA system TA149487

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 73273..73694
• Replicon: plasmid putative_pEcol1
• Accession: NZ_CP048440
• Organism: Escherichia coli strain NBRC 3301

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: GXM24_RS22905
• Protein ID: WP_001302184.1
• Coordinates: 73273..73431 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	73496..73694 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GXM24_RS22875	68670..69359	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
GXM24_RS22880	69546..69929	-	384	WP_001151527.1	relaxosome protein TraM	-
GXM24_RS22885	70262..70852	+	591	WP_001375132.1	transglycosylase SLT domain-containing protein	-
GXM24_RS22890	71148..71969	-	822	WP_001234417.1	DUF945 domain-containing protein	-
GXM24_RS22895	72088..72375	-	288	WP_000107542.1	hypothetical protein	-
GXM24_RS23150	72400..72606	-	207	WP_000547965.1	hypothetical protein	-
GXM24_RS23080	72676..73035	+	360	Protein_71	hypothetical protein	-
GXM24_RS22905	73273..73431	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	73496..73694	-	199	NuclAT_0	-	Antitoxin
-	73496..73694	-	199	NuclAT_0	-	Antitoxin
-	73496..73694	-	199	NuclAT_0	-	Antitoxin
-	73496..73694	-	199	NuclAT_0	-	Antitoxin
GXM24_RS22910	73706..74425	-	720	WP_000116348.1	plasmid SOS inhibition protein A	-
GXM24_RS22915	74422..74856	-	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
GXM24_RS22920	74911..76869	-	1959	WP_000117173.1	ParB/RepB/Spo0J family partition protein	-
GXM24_RS22925	76935..77168	-	234	WP_000005990.1	DUF905 family protein	-
GXM24_RS22930	77225..77764	-	540	WP_000290801.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002483 Escherichia coli K-12 Conjugative plasmid TA006145, TA006369, TA020275 1..99159 0	inside	Conjugative plasmid	-	-	1..98786	98786

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T149487 WP_001302184.1 NZ_CP048440:c73431-73273 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T149487 NZ_CP064118:c2078232-2077774 [Yersinia pestis]
ATGACGCTATACCGCATTGTTATGCGCCGCTATCTTGCATCGGCATGGACTGGTTACGGCGCTGAAATCTATGGTGGTCG
TTGGAATCATAAAGGCCATGCTGCCATCTATTTAGCCAGCAGTATTTCATTGGCTATGCTCGAAACACTGGCACATATTC
AGGACAGTTCAACACTCAGCGAATTTGAATTATTCCAAATTGAAATCAGTGACAGCAGTATTATGTTGCTACAGCCGCAG
GACTGGCCCACGGATTGGCGTAGCGATCCGGCACCGGTGGCAACGATGGATGTCGGCACCGAGTGGCTGGAATCAGAATC
CTCGGTAGGCTTACTGGTCCCGTCCACGTTGGTGCCGTCAGAAAACAACCTACTGGTCAATCCTCATCACAAAGATTTTC
CAGCGTGTTTGGCCAGCGTCAAACCGCTCTCTTTTGTCTTTGATCCACGGTTAAAATAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT149487 NZ_CP048440:c73694-73496 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin