Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) ccdAB/RHH-MazF
Location 46340..46865 Replicon plasmid F
Accession NC_002483
Organism Escherichia coli K-12

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag HXF02_RS00205 Protein ID WP_001159868.1
Coordinates 46560..46865 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag HXF02_RS00200 Protein ID WP_000813634.1
Coordinates 46340..46558 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXF02_RS00185 (D616_p97019) 41496..42395 + 900 WP_000963206.1 nucleotide-binding protein -
HXF02_RS00190 (D616_p97099) 42445..44670 - 2226 WP_000698737.1 P-loop NTPase fold protein -
HXF02_RS00195 (D616_p97093) 44672..45760 - 1089 WP_000952217.1 transcriptional repressor PifC -
HXF02_RS00200 (D616_p97106) 46340..46558 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HXF02_RS00205 (D616_p97105) 46560..46865 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
HXF02_RS00210 (D616_p97090) 46866..47672 + 807 WP_000016982.1 site-specific integrase -
HXF02_RS00215 (D616_p97092) 48446..49201 + 756 WP_000852146.1 replication initiation protein RepE -
HXF02_RS00220 (D616_p97089) 49789..50955 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid - - 1..99159 99159


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T6145 WP_001159868.1 NC_002483:46560-46865 [Escherichia coli K-12]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T6145 NC_002483:46560-46865 [Escherichia coli K-12]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT6145 WP_000813634.1 NC_002483:46340-46558 [Escherichia coli K-12]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT6145 NC_002483:46340-46558 [Escherichia coli K-12]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T6109 uncultured bacterium

100

100

1
T10064 Dickeya dadantii 3937

36.275

100

0.366
T190 Escherichia coli O157:H7 str. EDL933

35.577

100

0.366
T10018 Escherichia coli O157:H7 str. Sakai

35.577

100

0.366
T10099 Escherichia coli Nissle 1917

35.577

100

0.366

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT6109 uncultured bacterium

100

100

1

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References

(1) Katsuhiko Kamada et al. (2003) Crystal structure of the MazE/MazF complex: molecular bases of antidote-toxin recognition. Molecular Cell 11(4):875-84. [PubMed:12718874] 3D_structure

(2) Tiffany M Halvorsen et al. (2022) Comparison of Kill Switch Toxins in Plant-Beneficial Pseudomonas fluorescens Reveals Drivers of Lethality, Stability, and Escape. ACS Synthetic Biology 11(11):3785-3796. [PubMed:36346907]


3D_structure protein structure literature